FABRICATION OF POLYVINYLPYRROLIDONE (PVP) - GREEN TEA EXTRACT (GTE) PARTICLE COMPOSITE USING ELECTROSPRAYING METHOD FOR DRUG DELIVERY SYSTEMS

• Main Author: (NIM : 20215040), KAMARUDDIN
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/22746
• Institution: Institut Teknologi Bandung

Tea (camellia sinensis) is a kind of plants which is widely consumed as beverage. Delight in consuming tea offers lots of benefit to our health. One of which is recommended is green tea. Besides its taste freshening, green tea contains polyphenol compound that consists of high antioxidant properties compared to other tea. Else, the dominant compound in green tea is catechin this catechin has other compound derivatives; those are epigallocatechin-3-gallate (EGCG), epicatechin-3-gallate (ECG), epicatechin (EC), and epigallocatechin (EGC) which are also capable of anti-bacteria, anti-microbe and preventing the act of growing cancer cells yet it is only 0.2 – 2.0 % of catechin that can be absorbed when taken orally. It is because molecule solubility and weight in the tea are relatively high, owing to the fact that the applying of high nanotechnology is essential. Electrospray method is one of methods to produce morphology materials from micrometer to nanometer. This method promises to have the best of producing particles in the size control and morphology which is easy, inexpensive and simple since it used active polymer and mixed-polynter in composite shapes. This research aims to get composite particles of green tea extract (GTE) using polyvinylpyrrolidone (PVP) as matrix to improve solubility of the tea for drug delivery system. In this case, PVP was served as carrier media and GTE was the active component or delivered-drug. GTE was obtained by synthesizing Green Tea using maceration technique and tested qualitatively by TLC. The result showed that GTE contained phenol compound, then PVP and GTE were prepared in solution for electro spray method with specified parameters. There was significant effect on the size of tension or potential given and characteristics of solution on spray mode seen. Spray mode of PVP solution in ethanol - deionized water mixed-solution (7:3 w/w) indicated that spray mode was single-jet and this was potential for producing particles in submicron size. But it changed from single-jet to multi-jet spray after GTE was involved. The yield of morphology characterized by scanning electron microscope (SEM) suggested that composite particles of PVP-GTE were spherical and they were distributed from micrometer to nanometer. The result of meting average diameter of composite particles showed that the higher GTE was involved the smaller the size of particle. Flow rate variations of PVP-GTE (6:4) indicated that the smaller the flow rate was the smaller the size of composite particles of PVP-GTE was produced. The size of composite particle of PVP-GTE indicated that there was compatibility with theory model ever-before. Fourier transform infra-red (FTIR) spectrum indicated that there was a shift in wave number of PVP after GTE was loaded successfully. Likewise, the pattern of the differential scanning calorimetry (DSC) and the x-ray diffraction (XRD) characterization showed that the loading of ETH into PVP-ETH successfully done. The result of antioxidant activity assay using 2,2-diphenyl-1-picrylhydrazyl (DPPH) method showed that composite particles of PVP-GTE had high antioxidant activities. Besides that, composite particles of PVP-GTE had higher antioxidant activities than pure GTE. In-vitro assay of GTE showed that the extraction flow of PVP after made in composite particles of PVP-GTE was higher than pure GTE and the smaller the diameter was the faster GTE was released. Thus, the making of composite particles of PVP-GTE using Electro spray method provides potential in development for drug delivery system application.