PRELIMINARY STUDIES ON UTILIZATION OF SERICIN SILKWORM COCOON FOR NANOFIBER PREPARATION BY ELECTROSPINNING
The main components of silkworm cocoons are fibroin and sericin; the first one is <br /> <br /> <br /> used for silk fibers while the second one is often removed in degumming process. <br /> <br /> <br /> Previous research showed that sericin can be utilized a...
Saved in:
Main Author: | |
---|---|
Format: | Theses |
Language: | Indonesia |
Online Access: | https://digilib.itb.ac.id/gdl/view/22809 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Institut Teknologi Bandung |
Language: | Indonesia |
Summary: | The main components of silkworm cocoons are fibroin and sericin; the first one is <br />
<br />
<br />
used for silk fibers while the second one is often removed in degumming process. <br />
<br />
<br />
Previous research showed that sericin can be utilized as biomaterials with a wide <br />
<br />
<br />
range of applications such as recycled biomaterials, medical biomaterials, <br />
<br />
<br />
membranes, textile fibers with special properties and cosmetics. Unfortunately, in <br />
<br />
<br />
silk industries in Indonesia, sericin has not been utilized yet. Therefore, this study <br />
<br />
<br />
aims to open a new application of sericin in the field of textile by preparing <br />
<br />
<br />
sericin nano fiber using electrospinning technique. The first stage of this work <br />
<br />
<br />
was the extraction of silkworm cocoons using high temperature and pressure, then <br />
<br />
<br />
the preparation of sericin powder by lyophilization method and finally the <br />
<br />
<br />
formation of nano fibers by electrospinning. The extraction of silkworm cocoons <br />
<br />
<br />
was carried out in a high temperature-high pressure dyeing machine, which <br />
<br />
<br />
produced sericin solution with a concentration of 38.16 mg/mL. The sericin <br />
<br />
<br />
powder was produced by freeze-drying method with a yield of 15.08%. It was <br />
<br />
<br />
found that the sericin could not be used as a single component in producing nano <br />
<br />
<br />
fibers by electrospinning. However, nano fibers can be formed when sericin was <br />
<br />
<br />
mixed with other polymers, such as polyvinyl alcohol (PVA) and chitosan. In <br />
<br />
<br />
PVA-sericin solution, sericin was dissolved in trifluoro acetic acid (TFA) with <br />
<br />
<br />
concentration varied from 16 to 20% w/w. Each sericin solution was mixed with <br />
<br />
<br />
10% PVA w/w in the ratio of 50:50. The voltage of electrospinning used was 25 <br />
<br />
<br />
kV, the Taylor cone distance (TCD) was 10 cm and the flow rate was 0.1 μL/h. <br />
<br />
<br />
Higher sericin concentration resulted in good PVA-sericin fiber without melt. In <br />
<br />
<br />
the preparation of chitosan-sericin solution, chitosan and sericin was dissolved in <br />
<br />
<br />
TFA with a composition of 1:1, 1:3 and 1:5 (% w/w). These solutions were <br />
<br />
<br />
electrospinned with a voltage of 30, 32.5 and 35 kV; flow rate of 0.4 and 0.6 <br />
μL/h; TCD of 10 and 15 cm. The clearest form of fibers and without melt was <br />
<br />
<br />
obtained at a concentration of 10% PVA : 20% sericin (50:50) with <br />
<br />
<br />
electrospinning condition parameters are voltage 25 kV, TCD 10 cm and a flow <br />
<br />
<br />
rate of 0.1 μL/hour. The optimum chitosan-sericin fiber characteristics having the <br />
<br />
<br />
smallest average diameter and variation coefficient was achieved when the <br />
<br />
<br />
composition of chitosan-sericin was 1:3 (% w/w) and the voltage of the <br />
<br />
<br />
electrospinning was 30 kV, the flow rate was 0.4 mL/h and the TCD was 15 cm. <br />
<br />
<br />
It can be concluded that sericin can be used as a material for preparing nano fibers <br />
<br />
<br />
by electrospinning. |
---|