Polymorphisms study of Plasmodium falciparum EBA-175 Region IV-V from Timika Papua Isolate
Erythrocyte-Binding Antigen 175 (EBA175) Plasmodium falciparum is one of the antigen candidates for the development of a blood-stage vaccine. EBA-175 becomes an important antigen candidate because it has been studied plays a role in the process of invasion of Plasmodium and has an area that can inte...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/23037 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Erythrocyte-Binding Antigen 175 (EBA175) Plasmodium falciparum is one of the antigen candidates for the development of a blood-stage vaccine. EBA-175 becomes an important antigen candidate because it has been studied plays a role in the process of invasion of Plasmodium and has an area that can interact with Glycophorin A in erythrocytes. The EBA-175 gene consists of several regions where regions I and VI are known to have no binding activity. Region II has been widely researched and identified while the region III-V is unclear. Research shows that RIII-V forms better protection than RII so it can be a more effective vaccine candidate. According to the literature, the IV-V region is highly conserved and is an ideal area as an antigen candidate for vaccines but no studies have been done to look at Plasmodium Falciparum RIV-V polymorphisms in Indonesia. This study is a preliminary study for the development of an effective and efficient malaria vaccine. In this study, the EBA-175 DNA template was isolated from a clinical sample of malaria sufferers in Timika, Papua. Furthermore EBA175 RIII-V (~ 1600 bp) in PCR is then cloned to the pGEM-T easy vector (~ 3000 bp) in Eschericia coli TOP 10F '. Recombinant plasmid was analyzed restriction by using PstI enzyme. Restriction results obtained by a fragment of ~ 4600 bp which indicates that cloning pGEM-T_EBA-175 has been successfully performed. Alignment of 37 sequencing results from 11 samples showed mutations, deletions and insertions in some nucleotides resulting in polymorphisms in the EBA175 RIV-V gene originating from the isolate of Timika Papua. Sustainable areas are found in nucleotides 3429 to 3493, 3567 to 3623, and 3792 to 3742. The results of this study indicate the importance of polymorphism studies in the development of antimalarial vaccines. |
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