#TITLE_ALTERNATIVE#

• Main Author: ILHAM TOMAGOLA (NIM: 20714316), M.
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/23075
• Institution: Institut Teknologi Bandung

The definition of liver fibrosis is an excessive accumulation of the number of the extracellular matrixes and is usually the final stage of chronic liver injury. Liver damage causes the hepatic stellate cells to become more active and lead to increase synthesis of extracellular matrices, especially collagen. Potential antifibrotic therapies are aimed at inhibiting activation of fibrogenic cells, inducing apoptosis of activated hepatic stellate cells, and/or preventing deposition of Extracelullar matrixes proteins. Curcumin, a natural compound has been known possessing activity for liver fibrosis. Recently, curcumin has been indicated as a potential treatment for liver damage through mediation of various signaling pathways. It decreased the expression of pro-inflammatory mediators, inhibited activation of hepatic stellate cells in vitro by reducing cell proliferation, inducing apoptosis and suppressing extracellular matrixes gene expression. Numbers of approaches have been made to improve effectiveness of curcumin as antifibrosis. One of them is nanoparticle approach by conjugation between curcumin and gold based on green chemistry concept. Gold nanoparticle as carrier is inert, non toxic and highly accumulated in the liver. The formation of conjugation between curcumin and gold done in this research is expected to deliver curcumin specifically to the liver to improve the effectiviity through changing the distribution of drug only directed to target organ. The main aim of drug targeting is to maximize the drug accumulation in the target site and to minimize the adverse effect. Curcumin-gold nanoparticle was prepared by varying parameters such as pH of curcumin, curcumin:HAuCl4 ratio, speed and the duration of agitation process. Successful conjugation was indicated by color alteration from yellow to pink. Curcumin-gold nanoparticle was characterized by UV-visible spectrophotometry, infrared spectrophotometry, differential scanning calorimetry, x-ray diffraction, particle size, index of polidispersity and morphology of particle. To determine the stability of curcumin after reaction, an antioxidant assay was performed on gold-curcumin nanoparticles using DPPH method. Physical and chemical stability of curcumin-gold nanoparticle also performed for a month at 5oC, room temperature and 40oC. Biological <br /> <br /> v <br /> <br /> activity of curcumin-gold nanoparticle was performed in vitro using NIH/3T3 cells. The optimum condition for curcumin-gold nanoparticle was at pH 9.3 stirring speed 1000 rpm for 2 hours and molar ratio of curcumin to HAuCl4 1.5:1. The morphology of curcumin-gold nanoparticle is spheric with the size of 50 nm to 200 nm. The antioxidant activity of gold-curcumin nanoparticles is very strong and increases significantly compared to free curcumin. The curcumin-gold nanoparticle showed slightly increased in the particle size after stored at 5oC, room temperature and 40oC, but slightly reduced the curcumin degradation for a month. Incubation of NIH/3T3 cells with curcumin-gold nanoparticle at concentration of <1 &#956;g/mL preserved the cell viability and reduced the level of collagen protein significantly. In addition and more importantly, at concentration 1 &#956;g/mL, curcumin-gold nanoparticles demonstrated antifibrosis effect in vitro through inhibiting collagen production in NIH/3T3 cells as compared to its free curcumin. However, to study the targeting property to the liver, futher in vivo study is absolutely required.