IDENTIFICATION AND CHARACTERIZATION OF AMINOCYCLOPROPANE CARBOXYLIC ACID SYNTHASE (ACS) AND AMINOCYCLOPROPANE CARBOXYLIC ACID OXIDASE (ACO) GENE FAMILY IN BANANA GENOME A (Musa acuminata) AND B (Musa balbisiana)

• Main Author: Luki Nakasira (NIM : 10611078), M.
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/23090
• Institution: Institut Teknologi Bandung

Banana is one of the vital and popular food in the world. The one that we consumed was originated mainly from hybridization of two different banana, Musa acuminata (A genome) and Musa balbisiana (B genome). Banana is a climacteric fruit which depends on ethylene for its ripening. Aminocyclopropane Carboxylic Acid Synthase (ACS) and Aminocyclopropane Carboxylic Acid Oxidase (ACO) are the enzymes needed for Ethylene Biosynthesis. With the knowledge of ACS and ACO, we can understand and modified fruit ripening. Therefore, this study aims to be able to characterized the structure and function of ACS and ACO family on Musa acuminata and Musa balbisiana. Banana genome data acquired from Banana Genome Hub website and then the domain annotated using Pfam. The Annotated data used for further characterization of Structure and Function as follows: length, weight, and molecule isoelectric point (pI) measured using Vector NTI; signal peptide and transmembrane domain by SignalP 4.1; subcellular protein localization by WoLF PSORT and Plant-mPloc; phylogenetic analysis by IQTREE and conserved residue by multiple sequence alignment on MEGA7. Annotated domain of ACO, 2OG-FeII_Oxy (PF03171) and DIOX_N (PF14226) showed that there are 15 ACO in genome A and 9 ACO in genome B. Annotated domain of ACS, Aminotransferase_1_2 (PF00155) showed that there are 11 ACS in genome A and 13 ACS in genome B. The length of ACS sequences are around 440-917 AA. The length of ACO sequences are around 91-367 AA. The molecular weight of ACS are around 49-102 kDa. The molecular weight of ACO are around 10-40 kDa. Isoelectric Point for ACS and ACO variates between 4,6 (acid) and 9,3 (base). Most ACS and ACO didn’t have signal peptide and transmembrane domain. ACS and ACO proteins localized mainly in cytoplasm. Phylogenetic tree result showed that ACS and ACO proteins had high sequence similarity index. Electroforegram on banana fruit showed that ripening fruit genes were expressed. From this study, it can be concluded that ACS and ACO gene family in banana genome A and genome B had various structure, biochemical properties, function, and expressed on ripe banana fruit.