#TITLE_ALTERNATIVE#

• Main Author: FADHOLI (NIM : 11213018), MOCHAMMAD
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/23102
• Institution: Institut Teknologi Bandung

Gaharu (Aquilaria malaccensis) produces agarwood. Agarwood is used as raw materials for perfume, medicines and cosmetics. The intensity of gaharu trading made this plant included in threatened species, so that alternative technology were needed to overcome extinction of gaharu plants as well as to meet the market demand for gaharu, one of it was plant propagation by in vitro method. In this study, shoot culture was initiated on solid MS medium and acclimatized on ½ MS supplemented with 0,5 ppm BAP in TLC liquid medium. Propagation of shoots was done by using TIS RITA® bioreactor, by optimizing the time immersion of 15 minutes and 5 minutes every 4 hours. Results showed that weight of dry biomass of planlet increased 1.6 times in both treatments. However, there was no significant difference between treatments. While the growth rate of shoot growth in term of height and shoot multiplication as well as the content of secondary metabolite compound was highest on treatment of 15 minutes immersion. Metabolites detected in shoot culture were Phenol, 2,4-bis (1,1-dimethylethyl) -, Dibutyl phthalate, Eicosane, Stigmasterol, and Ethyl Oleate. In addition, an economic analysis between the TIS RITA® bioreactor and the bubble column in shoot production, showed that bubble columns was slightly more efficient (NPV Rp 3.208.745) than TIS RITA® (NPV value Rp 3.012.862) for 5 periods with payback period 3 years 5 months (bubble column) and 3 years 7 months respectively (TIS RITA®).