ISOLATION, SCREENING AND CHARACTERIZATION OF BIOSURFACTANT PRODUCING-BACTERIA FROM OIL RESERVOIR X, SOUTH SUMATERA FOR MICROBIAL ENHANCED OIL RECOVERY (MEOR) APPLICATION

• Main Author: SYUKRON (NIM 10413003), MUHAMAD
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/23178
• Institution: Institut Teknologi Bandung

Indonesia's oil production has continued to decline since 1995. It is not comparable to the increasing demand for oil every year. Therefore, it is necessary solution to increase the amount of oil production by using the optimization approach of oil recovery from the remaining reserves with Microbial Enhanced Oil Recovery (MEOR) technology. This technology utilizes microorganisms and bioproducts that are injected into the oil reservoir. One of the bioproducts that play an important role in MEOR applications is biosurfactant. This study focuses on isolation and screening for potential biosurfactant producer from crude oil sample of reservoir X, South Sumatera. The isolation stages was carried out at a temperature of 50°C using SMSS media with 0.1% (w/v) yeast extract and 2% (v/v) crude oil as a single carbon source. Of 31 isolate of bacteria were isolated sequential and screened by using qualitative parameters (Hemolytic Blood Assay, Oil Spreading Test) and semi-quantitative (Emulsification Index Test) have been successfully and selected biosurfactant-producing bacteria from thermophilic groups was isolate coded DS1 having the highest emulsification activity 65,19% with CMC value 157,5 mg/L and able to reduce IFT between oil and water from 27,2 dyne/cm to 19,8 dyne/cm. The results of a phylogenetic analysis based on 16S rRNA gene showed that the isolate DS1 has identitied as Bacillus licheniformis species and subsequently named as Bacillus licheniformis DS1. Characterization of functional groups by using FTIR revealed that biosurfactant produced was lipopeptide and growth-associated metabolite type. In addition, the crude of biosurfactant has good stability in maintaining emulsification activity at high temperatures up to 120oC (for 15 minutes), salinity up to 10% NaCl (w / v), and in the wide range of pH 4-10 based on surface response analysis with Box-Behnken design model. According to this study, it has been estimated that Bacillus licheniformis DS1 and its biosurfactant can be applied for MEOR applications