DETECTION OF INFECTIOUS MYONECROSIS VIRUS (IMNV) ON WHITE SHRIMP (Litopenaeus vannamei) USING REVERSE TRANSCRIPTION LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (RT-LAMP)
White shrimp or vannamei shrimp is one of the most desirable and economical cultivation in Indonesia. Infectious Myonecrosis Virus (IMNV) is an RNA virus that can infect white shrimp causing necrosis of muscle tissue. IMNV infection leads to a decrease in production rates because it often leads to d...
Saved in:
| Main Author: | |
|---|---|
| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/23396 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | White shrimp or vannamei shrimp is one of the most desirable and economical cultivation in Indonesia. Infectious Myonecrosis Virus (IMNV) is an RNA virus that can infect white shrimp causing necrosis of muscle tissue. IMNV infection leads to a decrease in production rates because it often leads to death in shrimp. Shrimps do not have a natural immune system to prevent infection. One attempt to prevent the spread of IMNV infection through early detection methods on uninfected and infected shrimps. Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) is a fast and sensitive amplification method that works in isothermal conditions with the principle of strand displacement. This study aims to detect IMNV and to examine the sensitivity of primers designed in RT-LAMP reactions to IMNV that infect white shrimp or vannamei shrimp (Litopenaeus vannamei). The RT-LAMP reaction was performed with three stages, i.e. preheating stage at 95°C for 5 min, then incubation temperature of 63°C for 45 min, and termination stage at 80°C for 5 min. The results obtained in this study, the RTLAMP reaction with primers designed to amplify the template, is indicated by the color change in the RT-LAMP yield solution after SYBR Green added, but the same thing also occurs in the control solution without being given a template containing only primers so it is suspected to occur false positive due to primer dimer. Test results for the various combinations of primers in the RT-LAMP reaction without the use of the template show no change of color in the reaction without the primary pair of loops. These results indicate that the primers designed can be used in the detection of IMNV in the RT-LAMP reaction. |
|---|