THE EFFECTS OF STATIC ELECTRIC FIELD ON MOTILITY OF MICE (Mus musculus) SPERMS THROUGH VELOCITY AND SPEED DISTRIBUTIONS

• Main Author: (NIM : 90214015), NURMASYITAH
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/23581
• Institution: Institut Teknologi Bandung

The radiation of electromagnetic waves affect the quality of sperms, it decreases the sperm motility and viability. Mice (Mus musculus) can be used as experimental animal because the reproductive system is almost the same as <br /> <br /> <br /> <br /> <br /> human. The purpose of this study is to determine the effect of static electric field on motility and viability of mice sperm in an ex vivo condition through the velocity and speed distributions at time t = (1, 15, 30, 45, 60, 90, and 120) minutes for controls (without electric field) and t = (1, 15, 30, 45, and 60) minutes when affected by electric field. The mice sperm is diluted with NaCl liquid 0,9 %. This liquid is then put in a hemocytometer that is placed between two iron metal plates which are connected to a DC voltage source. The distance between the two plates is d = 7.5 cm and the voltage is varied (&#916;V = 0 V (controls), &#916;V = 12.5 V and &#916;V = 25.0 V). Sperm motility is observed using a microscope flourescence then recorded using NIS Elements software. Sperm without electric field (&#916;V = 0) had viability 45 minutes, it was longer than the sperm affected by electric field (&#916;V = 12,5 V and &#916;V = 25,0 V) that had viability for 30 minutes. Sperms move almost randomly in all directions when without electric field, but the sperms tend to move to the positive plate under the influence of electric field. The quality of normal sperm is characterized by 25% of total sperm in fast-zigzag movement. This research of sperms showed that speed of fast-zigzag movement was more than 37 &#956;m/s. The most probable speed (vmp), the average speed ( v ), and root <br /> <br /> <br /> <br /> <br /> mean square speed ( rms v ) satisfied the relation of mp rms v (formula) v (formula) v . The maximum speed decreased with increasing time of observation.