#TITLE_ALTERNATIVE#

• Main Author: MUHLISA MUS (NIM: 20715026), NURUL
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/23600
• Institution: Institut Teknologi Bandung

Immunomodulators are compounds that can either stimulate or suppress the body's immune response and can be used to treat in autoimmune or immunodeficiency patients such as rheumatoid arthritis (RA). This study aims to examine the immunomodulator effect of melinjo seeds (Gnetum gnemon L.) which is contains many stilbene that are thought have effect in RA. The active compounds of melinjo seeds were extracted in 55% ethanol by maceration method. This study aims to prove the activity of melinjo seed as an immunomodulatory agent and its effect on animal models of RA. Immunomodulatory activity of extract was determined through carbon clearance test, hemaglutination assay (HA) and cytokine level. Meanwhile, antiarthritis activity of the test extract was determined by testing on animal models of adjuvant induced arthritis (AIA) by injecting CFA intraplantar. The effect in RA rat model was observed for 28 days based on measurement of foot thickness, joint diameter, body weight, arthritis score and TNF-a levels and histologic observation of the joints. Safety long-term use was observed by measuring GPT activity, albumin and total protein levels. The test results showed that all three doses of melinjo seed extract (50, 100, and 200 mg/kg bw) showed immunosuppressing activity on phagocytic index and organ index (liver, spleen, thymus gland). In a test of a second specific immune response the dose of melinjo seed extract (100 and 200 mg/kg bw) may decrease antibody titers. However, it does not affect the levels of IFN-&#611; and IL-2. In the test of RA rats, the highest dose of extract (200 mg/kg bw) inhibited leg swelling (34.65%) significantly (p<0.01), decreased joint diameter by 41.3% (p<0.1), decreased the arthritis score significantly (p<0.01) but did not significantly lower TNF-k levels. The administration of the extract (200 mg/kg bw) for 28 days did not affect liver function indicated from GPT activity, total albumin and protein levels that no different compared to control. Based on the results of this study, the three doses of melinjo seed extract are immunosuppressant and can decrease the symptoms of arthritis.