#TITLE_ALTERNATIVE#

• Main Author: WAHYUNI NIM: 20715314, NURUL
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/23605
• Institution: Institut Teknologi Bandung

Background and purposes: The human body has the ability to produce <br /> <br /> antioxidants as its self-defense against the attacks of free radicals. Environmental <br /> <br /> conditions as well as an unhealthy lifestyle resulted in the need of other <br /> <br /> antioxidants which inhibit the increasing of free radical activity. Honey is one of <br /> <br /> the beekeeping products that have the ability as an antioxidant. The purposes of <br /> <br /> this study were to determine antioxidant activities of 20 extracts of honey derived <br /> <br /> from 3 species of bees namely Apis dorsata, Apis cerana and Trigona sp. by <br /> <br /> determining IC50 of DPPH and EC50 of CUPRAC, determine phenolic and <br /> <br /> flavonoid content, analyze correlation of phenolic and flavonoid content with IC50 <br /> <br /> of DPPH and EC50 of CUPRAC as well as correlations between them. Methods: <br /> <br /> Honey was extracted by reflux method using three solvents with increasing <br /> <br /> polarity, ie n-hexane, ethyl acetate and ethanol. In addition directly extraction <br /> <br /> using ethanol was carried out. Determination of antioxidant activity by DPPH and <br /> <br /> CUPRAC methods. Determination of total phenolic content using Folin-Ciocalteu <br /> <br /> reagent and flavonoid content by Chang’s method. Correlation of total phenolic <br /> <br /> and flavonoid content with IC50 of DPPH and EC50 of CUPRAC and the <br /> <br /> correlation between two antioxidant methods were analyzed by Pearson’s method. <br /> <br /> Result: All honey extracts both extraction by increasing polarity solvents and <br /> <br /> directly extraction using 96% ethanol had IC50 of DPPH value less than 50 &#956;g/mL <br /> <br /> (except ethyl acetate extract of Cerana honey from Lombok). Total phenolic and <br /> <br /> flavonoid content of Dorsata Sumbawa honey extract gave significantly negative <br /> <br /> correlation to their IC50 of DPPH and EC50 of CUPRAC. Conclusion: All honey <br /> <br /> extracts (except ethyl acetate extract of Cerana honey from Lombok) were <br /> <br /> categorized as very strong antioxidant. Phenolic and flavonoid compounds were <br /> <br /> contributor major of antioxidant activity of Dorsata Sumbawa honey by DPPH <br /> <br /> and CUPRAC methods. DPPH and CUPRAC methods did not show linear <br /> <br /> antioxidant activities for all honey extracts.