DETECTION OF BLOOD DISEASE BACTERIUM (BDB) IN Drosophila melanogaster BY USING FLOURESCENCE IN SITU HYBRIDIZATION (FISH)

• Main Author: Sativa , Oriza
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/23642
• Institution: Institut Teknologi Bandung

Indonesia is one of the biggest banana producer in the world. Recent years banana production si decreased, one of the major causes is blood disease infection which caused by Blood Disease Bacterium (BDB). Blood disease dispersion can be caused by insects as its vector. Drosophila melanogaster was suspected as dispersal vector of BDB. Purpose To analyse Blood Disease Bacterium presence which was infected to D. melanogaster through oral administration route. BDB used in the experiment was taken from pure culture belongs to School of Life Science and Technology ITB. The culture was then activated in TZC (Tetra Zolium Chloride) medium, from those culture we obtained standard curve and growth curve. Infection was done by mixing BDB with D. melanogaster’s food. BDB presence assessment in D. melanogaster was done using Florescence In-Situ Hybridization (FISH) 24 hours after infection. BDB that was infected into D. melanogaster, was in log phase (OD = 0.4). Mortality percentage of D.melanogaster that was infected by BDB compared to control group was 17.33% and 34.33%, this difference was statistically significant (p<0.05). BDB was higher at D. melanogaster’s body surface compared to the inner body. Immune system caused BDB found inside the body of D. melanogaster fewer. The higher amount of BDB on the body surface caused by direct contact of D. melanogaster with their food. From this experiment, we conclude that BDB colony in infected D. melanogaster was higher at the body surface than inside the body.