DETERMINATION OF DINITRO-ORTHO-CRESOL BY MOLECULARLY IMPRINTED POLYMERS SOLID PHASE EXTRACTION (MISPE-HPLC) METHODS
The globalization of food trade has spread to many countries, and its presence is inevitable. Viewed from the aspect of food security, globalization can enlarge and expand the possibility of the emergence of hazards contained in food consumed. Therefore, the demand for food security guarantees conti...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/23765 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | The globalization of food trade has spread to many countries, and its presence is inevitable. Viewed from the aspect of food security, globalization can enlarge and expand the possibility of the emergence of hazards contained in food consumed. Therefore, the demand for food security guarantees continues to increase in line with increasing public awareness about the importance of food health consumed. Pesticides are widely applied to food production for preventing crop damage from insects, mites, mice, etc. Dinitro-ortho-cresol (DNOC) is a pesticide compound commonly used as herbicides, larvacides, and insecticides in agriculture. DNOC is a toxic compound when exposed to humans and it can cause nausea, stomach disorders, anxiety, excessive burning, etc. Based on these hazards, the presence of these compounds in food must be identified and analyzed. Several methods have been widely used for the preparation of DNOC samples and other pesticide compounds in general such as liquid-liquid extraction and QuEChERS. The method still has deficiency that can not be specific to one compound. The developed method in this research was solid phase extraction method using molecularly imprinted polymers as a solid sorbent. This method is chosen because of the low cost, easy preparation, stable, repeatable use without loss of activity, high mechanical strength, and resistance to heat and pressure. The purpose of this research is to synthesize MIP functional material which can be used as SPE column filler. The obtained MIP was used for the preparation of a sample of DNOC pesticides in tomatoes. MIP for DNOC was prepared using methacrylic acid (MAA) as its functional monomer, ethylene glycol dimethacrylate (EGDMA) as a crosslinker, benzoyl peroxide (BPO) as an initiator, and acetonitrile as a porogen. The adsorption or retention characterization of this material was carried out by batch and dynamic method. The characterization of the material showed that the optimum adsorption conditions were found at pH 5, contact time at 30 minutes with adsorption capacity of 137 mg/g, the eluant of desorption used methanol:acetate (75:25). The adsorption process follows Sip's adsorption isotherm with a value of n 0.967 indicating that the adsorption occurs on homogeneous surfaces and monolayer. Reusability studies showed that the recovery of each cycle is 109.4; 108.8; 101.1 %. The obtained MIP was applied as a column filler for the DNOC preconcentration process from the tomato sample before being analyzed using HPLC. The DNOC concentration obtained in tomato sample was found 0.65 mg/kg with recovery 87.17 % which is within the tolerable range of 80-110%. |
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