Study of Textile Dyes Biodegradability Using Pure Laccase from

• Main Author: PUTRI WIDYASTUTI (NIM : 21114012), RAGIL
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/23778
• Institution: Institut Teknologi Bandung

Dyes in textile waste become one of the important components of pollution because it is difficult to degrade. The laccase enzyme derived from the white fungus Marasmiellus palmivorus proved effective in degrading several types of textile dyes. Enzyme is obtained by extracting mushrooms grown in SSF (Solid State Fermentation) media. The crude extract of laccase is purified by hydrophobic interaction chromatography and ion-exchange chromatography. The pure laccase is <br /> <br /> tested on a textile dye by the High Throughput Screening (HTS) method. The number of dye samples used is the reason for choosing this method. The dye samples used were 14 dyes of azo, anthrakuinon and disperse type. Sodium sulfate is added to a textile dye, pH 12.5 and heated in a temperature of 80oC to mimic the effluent. Rapid test was performed at room temperature using microplate titer with sample ratio and 1: 1 enzyme for 24 hours with dye concentration of 150 ppm. The <br /> <br /> samples observed were a mixture of dye and pure enzymes with specific activity of 2.49 U / mg. Screening of dye decolorization by M. palmivorus mushroom culture <br /> <br /> on PDA medium was also performed. Mushroom cultures were grown on PDA media that had been dyeed and incubated at room temperature for 2 weeks. The HTS method shows Procion Blue H-GN and Levafix Blue E-Ra Gran positively <br /> <br /> polarized by laccase. While the test using mushroom culture showed the results of 7 dye decolorized are Telon Blue AFN, Telon Red AFG, Silver N Isolation, Telon <br /> <br /> Blue BRL, Levafix Blue PN-3R, Procion Blue H-GN and Levafix Blau E-Ra Gran. The number of dyes that have been successfully dissolved by the culture of the <br /> <br /> fungus may be due to the absorption of the dye by the mycelial mycelia. Two positive colors degraded by laccase and mushroom culture measured the change in <br /> <br /> concentration based on the absorbance value using a spectrophotometer. The concentration of Levafix Blue E-Ra Gran dye drops to 86 ppm after 24 hours. The <br /> <br /> conclusion of this study is that the HTS method successfully demonstrated the decolorization properties of various textile dyes quickly and proven by changing <br /> <br /> the dye concentration after the test.