#TITLE_ALTERNATIVE#

• Main Author: KUSUMA PUTRI (NIM: 20714019), RIZKA
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/24060
• Institution: Institut Teknologi Bandung

Diabetes is one of the largest global health emergencies of the 21st century. <br /> <br /> Diabetes characterized by an increase in blood sugar levels beyond the normal <br /> <br /> value. Various therapies to lower blood sugar levels have been introduced, one of <br /> <br /> them by inhibiting enzymes involved in carbohydrate hydrolysis process such as <br /> &#945;-amylase which can delay glucose absorption. The population in Indonesia used <br /> <br /> plants as traditional drug for treatment diabetes, the example are Strobilanthes <br /> <br /> crispa L. Blume leaf, Spondias dulcis Soland ex. Park leaf, Foeniculum vulgare <br /> <br /> Mill. seed, Alstonia scolaris (L.) R.Br. stem bark, Blumea balsamifera (L.) DC. <br /> <br /> leaf, Dioscorea hispida Dennst. leaf, Ocimum sanctum L. leaf, Phaseolus vulgaris <br /> <br /> L. seed, Ceiba pentandra (L.) Gaertn stem bark, Morus alba L. leaf, Syzygium <br /> <br /> cumini Merr. seed and stem bark, Phyllanthus acidus (L.) Skeels fruit, <br /> <br /> Phyllanthus emblica L. leaf, Phyllanthus niruri L. herb, Plantago major L. leaf, <br /> <br /> Gardenia jasminoides J. Ellis leaf, and Guazuma ulmifolia Lamk. var. tomentosa <br /> <br /> K. Schum leaf. The purpose of this research was to verify the plants that have <br /> <br /> used traditionally as diabetes drugs with an in vitro approach for &#945;-amylase <br /> <br /> enzyme inhibitor. Extraction was done by reflux method using 96% ethanol as <br /> <br /> solvent. The extracts obtained were freeze dried and tested their activity for &#945;- <br /> <br /> amylase inhibition at a concentration of 500 &#956;g/mL. Ceiba pentandra (L.) Gaertn <br /> <br /> stem bark extract had the highest presentage of inhibition compared to other test <br /> <br /> samples with a value of 97,64±0,52%. IC50 values of extract of P. emblica L. leaf, <br /> <br /> S.cumini Merr. seed, S. cumini Merr. stem bark, C. pentandra (L.) Gaertn stem <br /> <br /> bark were 35,56±8,09 &#956;g/mL; 25,63±1,91 &#956;g/mL; 21,84±0,24 &#956;g/mL; 12,62±1,81 <br /> &#956;g/mL; and 3,01±0,77 &#956;g/mL respectively. Then C. pentandra (L.) Gaertn <br /> <br /> became the selected plant for futher testing. The C. pentandra (L.) Gaertn stem <br /> <br /> bark ethanol extract is fractionated by liquid-liquid extraction method using nhexane <br /> <br /> and ethyl acetate. IC50 values for the n-hexane fraction, ethyl acetate <br /> <br /> fraction, and water fraction of C. pentandra (L.) Gaertn. stem bark were <br /> <br /> 491.41±29.80 &#956;g/mL; 322.61±9.29 &#956;g/mL; and 26.49±1.07 &#956;g/mL respectively. <br /> <br /> The verification of 18 plants that traditional used for diabetes treatment was done, <br /> <br /> C. pentandra (L.) Gaertn. stem bark extract had the best activity compared with <br /> <br /> its fractions. Simplicia and extract of C. pentandra (L.) Gaertn. stem bark contain <br /> <br /> of alkaloids, flavonoids, condensed tannin, saponin, quinones and <br /> <br /> steroids/triterpenoids.