GLYCOSYLATED FLAVONOL FROM CRYPTOCARYA CRASSINERVIA LEAVES AND THEIR BIOACTIVITIES AGAINST MURINE LEUKEMIA P-388 CELLS

• Main Author: ALRAMADHAN WIJAYA NIM: 10513011, RULAN
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/24130
• Institution: Institut Teknologi Bandung

Cryptocarya is a genus from Lauraceae family which spread over Asia, Australia, America,and Africa. Cryptocarya has a higher level of evolution compared to other genera in the same family, so it produces more diverse secondary metabolites. Phytochemical studies show that Cryptocarya main secondary metabolites compound is in alkaloid, 2-piron, and flavonoid groups. Other secondary metabolites that had been found from Cryptocarya is stilben, lignan, <br /> <br /> <br /> <br /> fenil propanoid, kumarin, polyketide derivatives, amide derivatives, terpennoid, steroid, and carboxylic acid and its derivatives. There are some species of Cryptocarya which grows in Indonesia, Cryptocarya crassinervia for example. Based on previous studies, compounds from lignan, terpenoid, fenantren structured alcaloid, aporfin, and benziltetrahidroisokuinolin groups had been isolated successfully. However, the studies that had been done and reported is still limited on stem bark tissues. Meanwhile, studies on the content of secondarym metabolites on other tissues hasn’t been done and reported. Therefore, the study of the content of secondary metabolites in the leaf tissues is done to obtain other compounds. In this study, the isolation of secondary metabolites is done through several steps. Leaf powder of Cryptocarya crassinervia was macerated using MeOH as a solvent thus obtaining MeOH <br /> <br /> <br /> <br /> macerate. This macerate which contains chlorophyll, was isolated from the chlorophyll with chlorophyll separation technique using MeOH-water thus obtaining EtOAc extract. This extract wasa further fractionated and purified with multiple techniques of chromatography such as: vacuum liquid chromatography, radial chromatography, and gravitation column chromatography to obtain three pure compounds. These pure compounds were further characterized with 1D-NMR (1H-NMR and 13C-NMR) and 2D-NMR (HSQC dan HMBC). These three compounds were suggested as flavonoid compounds: afzelin (kaempferol-3-Orhamnoside) (1), quercitrin (quercetin-3-O-rhamnoside) (2), and kaempferitrin (kaempferol- 3,7-O-dirhamnoside) (3). Compounds 1 and 3 were first found on Cryptocarya. Meanwhile, 2 was already isolated before from the leaf and twig of C.alba. These compounds were tested on their sitotoxicity on murine cell of leukemia P-388 using MTT assay method. The results show that compound 1, 2, and 3 respectively has IC50 value of 15,43 &#956;g/mL, 56,62 &#956;g/mL, <br /> <br /> <br /> <br /> 62,54 &#956;g/mL. The conclusion of this result is the extract is ac