APPLICATION OF BIOSURFACTANT PRODUCED FROM PSEUDOMONAS AERUGINOSA FOR OIL RECOVERY AS A PRE-TREATMENT OF OIL CONTAMINATED SOIL ON SLURRY PHASE BIOREMEDIATION METHOD

• Main Author: Indriana ( NIM : 15312047), Sandy
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/24196
• Institution: Institut Teknologi Bandung

Storage, transportation, and treatment of petroleum oil are often causing soil and soil water contamination. High concentration of hydrocarbon and toxic compound such as heavy metals contained in petroleum oil are heavily affecting the condition of nature and human health. Some more environmental friendly method of degrading hydrocarbons are being considered, such as oil recovery and remediation using biological process. This study was aimed to determine the effect of biosurfactan addition from Pseudomonas aeruginosa and petrophilic consortia in oil recovery and hydrocarbon degradation. Soil contaminated soil was obtained from contaminated soil around oil well in Grobogan, Jawa Tengah with the initial parameter of TPH 20,92%. Oil recovery study was done with three surfactants addition variations, which are biosurfactants (PS), surfactants Tween 80 (TW), and no surfacants addition as control (KT). Surfactant was added into oil contaminated soil with the ratio of 6:1. Variations of shaking time and surfactants concentration were also made with variations of shaking time are 6, 12, 18, 24, and 30 hours, variations of biosurfactant or surfactant concentrations are 3%, 5%, 7%, and 10%. Bioremediation was done using the addition of unidentified mixed culture and biochar. Reactor conditions had been made so that it met the criteria of bioremediation optimum parameters. The addition of biosurfactant increases volume of recovered oil significantly.The addition of unidentified mixed culture showed the increment of TPH removal efficiency, with the addition of unidentified mixed culture alongside with biochar showed the best result for bioremediation. However, further inspections are needed regarding additional energy requirement due to biochar addition.