ISOLATION OF SECONDARY METABOLITES FROMCOLLETOTRICHUM QUEENSLANDICUM, AN ENDOPHYTIC FUNGUS OF ARTOCARPUS CHAMPEDEN PLANT

• Main Author: PARIS MATAPUTUN (NIM:20515038), SAN
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/24202
• Institution: Institut Teknologi Bandung

Endophytic fungi are commonly found in plant tissue systems such as roots, stems, twigs and leaves without affecting host plants negatively for a period of time. In addition to being involved in ecosystem balance control, endophytic fungi also produce potential secondary enzymes and metabolites as lead compound candidates for the development of drug compounds. Artocarpus is a group of plants once reported as a host plant of endophytic fungi. Phytochemical studies of the genus ofArtocarpus show that these plants produce secondary metabolites such as stilbene, arylbenzofurans, flavonoids, terpenoids and steroids. In this study, isolation and characterization of secondary metabolites of endophytic fungi from A. champeden plants were performed. The purpose of this study was to obtain information on secondary metabolite content of endophytic fungi of A. champeden plants through several steps including isolationof endophytic fungi, fermentation, isolation of secondary metabolites and characterization of the isolated compounds. The isolation of endophytic fungi from A. chempeden begunby collecting of plant tissue samples, surface sterilization, inoculation on solid medium Potato Dextrose Agar (PDA), and followed by subculturing to obtain a single strain. This isolated single strain was identified in the Center for Biological Research InaCC LIPI Cibinong and identified as Colletotrichum queenslandicum. Fermentation of this endophytic fungus with large-scale was carried out in liquid medium Potato Dextrose Broth (PDB). Harvesting of fermented fungus was done on day 14 after fermentation because the secondary metabolites have been produced maximally based on the amount of extract mass obtained and the profile of thin layer chromatography analysis. Isolation of secondary metabolites was performed on C. queenslandicum mycelia and its liquid medium. In this study, the powder of miselia was macerated with methanol and evaporated to give 3.9 g of dry extract. The liquid medium (± 8 L) was partitioned with ethyl acetate (EtOAc). The EtOAc phase was evaporated and give 1 g of dry EtOAc extract. These two extracts were fractionated and purified using various chromatographic techniques, such as gravity column chromatography (KKG), gel filtration chromatography, and radial chromatography (KR). From the methanolextract,three pure compounds have been isolated and characterized namely; ergosterol (ergosta-5,7,22-trien-3&#946;-ol) (1), ergosterol peroxide (5,8-epidioxy-22E-ergosta-6,22-dien-3&#946;-ol) (2) and stearic acid (3). While in EtOAc <br /> <br /> <br /> extract,two pure compounds have been isolated, namely 3-acetic acid (4) and oleic acid (5). All structures of the compounds were determined based on NMR spectroscopy data (spectrum 1D-NMR and 2D-NMR). <br />