#TITLE_ALTERNATIVE#

• Main Author: YUNIARTI (NIM: 20714318), SARTIKA
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/24228
• Institution: Institut Teknologi Bandung

Background and Objectives: Kelor (Moringa oleifera Lamk.) is belonging to the Moringaceae family that has been used in the treatment of deseases traditionally, one of which is to treat diabetes mellitus. Diabetes mellitus is a public problems health in a worldwide. This study was designed to obtain efficacy data of kelor leaves water extract in diabetic mice induced with alloxan and to isolate a marker compound. Methods: Leaves were blended with the addition of aquades then filtered. The obtained filtrate was collected, then dried using a freeze dryer. Kelor leaves water extract was tested for antidiabetic activity for alloxan-induced mice. The mice were divided into four groups: the alloxan-induced group was given 0,3% Na CMC only, the alloxan-induced group and given glibenclamide with a dose of 0,37 mg/kg BW, another group was alloxan-induced and given the water extract of kelor leaves with dose 25 mg/kg BW and 50 mg/kg BW. Blood glucose levels were measured on day 7, 10, 14, and 21. Water extract of kelor leaves was fractionated by liquid-liquid extraction using ethyl acetate as solvent. Ethyl acetate fraction was sub-fractionated by classical coloumn chromatography. The isolate was purified by preparative thin layer chromatography and the purity testing was done using a single development thin layer chromatography and two-dimensional TLC. The isolate was characterized using TLC with specific visualization reagent and chemical shift reaction. Result: The result showed that extract of kelor leaves did not cause a decrease of blood glucose levels that significantly different compared to the sore group (p>0,05). Conclusion: The results of the isolation and the identification of water extract compound of kelor leaves based on identification with UV spectroscopy are suspected as an isolate F which is a flavonol, where there is an OH at C 3, C 7 and C 4 ' position, and the absence of ortho in OH-on ring B.