THE EFFECT OF METHYL JASMONATE ON GROWTH AND SESQUITERPENE FORMATION ON GAHARU (Aquilaria malaccensis Lam.) SHOOT CULTURE IN 1; 3; AND 5 WEEK CO-CULTURE

• Main Author: RAHMANI (NIM : 11213040), SILMI
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/24303
• Institution: Institut Teknologi Bandung

Aquilaria malaccensis Lam. is a plant which is producing resin (gaharu) that is <br /> <br /> <br /> <br /> <br /> <br /> widely used for perfume and medicine industries. The main compounds known to <br /> <br /> <br /> <br /> <br /> <br /> cause the fragrance of gaharu and have a very high selling value are sesquiterpene <br /> <br /> <br /> <br /> <br /> <br /> and chromone derivatives. These compounds are produced as an induced selfdefense <br /> <br /> <br /> <br /> <br /> <br /> response, one of them with a chemical compound. A. malaccensis shoot <br /> <br /> <br /> <br /> <br /> <br /> cultures can be one method of studying the mechanism of formation of secondary <br /> <br /> <br /> <br /> <br /> <br /> metabolites in gaharu by methyl jasmonate induction. The purpose of this study was <br /> <br /> <br /> <br /> <br /> <br /> to evaluate growth and sesquiterpene formation on A. malaccensis shoot culture due <br /> <br /> <br /> <br /> <br /> <br /> to the influence of variation methyl jasmonate concentration along with the length <br /> <br /> <br /> <br /> <br /> <br /> of co-culture time. This study was carried out by cultivating the culture of A. <br /> <br /> <br /> <br /> <br /> <br /> malaccensis in medium 1/2MS0 on thin layer culture (TLC) and adding methyl <br /> <br /> <br /> <br /> <br /> <br /> jasmonate at five different concentrations of 12.5; 25; 50; 100; and 150 &#956;M with <br /> <br /> <br /> <br /> <br /> <br /> long co-culture 1; 3; and 5 weeks. The parameters measured in this study were dry <br /> <br /> <br /> <br /> <br /> <br /> weight, amount of sucrose consumption, changes in conductivity value, and <br /> <br /> <br /> <br /> <br /> <br /> secondary metabolite content. The results showed that MejA with concentration of <br /> <br /> <br /> <br /> <br /> <br /> 12,5-150 &#956;M will inhibit growth and stress to A. malaccensis shoot culture with <br /> <br /> <br /> <br /> <br /> <br /> longer co-culture time. Increased concentrations of MeJA affect the amount and <br /> <br /> <br /> <br /> <br /> <br /> number of gaharu chemical compounds detected. Detection of sesquiterpenes, 3,5- <br /> <br /> <br /> <br /> <br /> <br /> di-tert-butyl-4-hydroxybenzaldehyde, at the end of the treatment of MejA 12.5; 25; <br /> <br /> <br /> <br /> <br /> <br /> 50; and 100 &#956;M is 1.18%; 1.29%; 1.59%; and 0.38% concentration. Treatment with <br /> <br /> <br /> <br /> <br /> <br /> all MeJA concentrations may induce the formation of chromone, 4H-1-benzopyran- <br /> <br /> <br /> <br /> <br /> <br /> 4-one, at week 3 of the co-culture, while shoots culture A. malaccensis with MejA <br /> <br /> <br /> <br /> <br /> <br /> 50 and 150 &#956;M have been able to induce chromone in the first week after treatment <br /> <br /> <br /> <br /> <br /> <br /> of which concentration increases with length of co-culture.