THE OPTIMAL NITROGEN AND PHOSPHORUS CONCENTRATION RATIO IN Botryococcus braunii CULTURE FOR GROWTH AND LIPID PRODUCTION

• Main Author: FREDY YAP - Nim: 11213006 , TUAH
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/24583
• Institution: Institut Teknologi Bandung

The aim of this research was to determine the effect of nitrogen and phosphorus concentration ratio (N:P) on growth kinetics, biomass productivity, total lipid, and fatty acid composition of Botryococcus braunii. N:P concentration ratio applied to B. braunii culture was 55:14 (control); 5:4, 350:4, 350:44, 350:94, 350:164, 510:4, 510:44, 510:94, and 510:164. The culture was aerated with a flow rate of 1.5 liters per minute and was irradiated using fluorescent lamps with a light intensity of 5,200 16:8 hours (light:darkness). The results indicated that the largest specific growth rate B. braunii in Chu-13 modification medium resulted when the concentration ratio of N:P was (350:164) (KNO3 2.525 mg / L and K2HPO4 920.5 mg / L) with &#956; = 0.25 ± 0.02 g of cells day-1), the lowest specific growth rate resulted when the concentration ratio of N:P was (55:14) with &#956; = 0.16 ± 0.02 g of cells day-1. The largest biomass productivity was obtained 58.2 ± 0.4 mg (L days)1 when the concentration ratio of N:P as much as 350:4 and the largest total lipids 15, 14 ± 0, 67% was produced when the concentration ratio of N:P was 5:4. Biomass productivity and total lipid were increased 1.12 and 4.69 folds from control at N:P concentration ratio 350:4 and 5:4. N:P concentration ratio 5:4 is the optimal ratio used to produce lipids with productivity of lipid was 8.2 mg (L day)1. The highest palmitate, oleic, stearic, and linoleic acid percentage resulted when the concentration ratio of N: P (350: 44), (55:14), (5: 4), (350: 164) were 26.69%; 31.22%; 17.77%; and 21.43% respectively. <br />