Effects of Benzylaminopurine and Naphthalene Acetic Acid on In vitro Axillary Shoots Growth of Purple Sweet Potato (Ipomoea batatas (L.) Lam cv. Ayamurasaki)

• Main Author: ALPIANI (NIM : 10613023), VINA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/24639
• Institution: Institut Teknologi Bandung

Purple Sweet Potato cultivar Ayamurasaki, a type of sweet potato was introduced from Japan and has been planted in several regions in Indonesia. Naturally sweet potato propagation is conducted through stem cuttings. In this study, propagation of Purple Sweet Potato is implemented by in vitro culture using nodal cuttings. The study aimed to determine the effect of giving benzyl amino purine (BAP) with or without naphthalene acetic acid (NAA) for axillary shoot growth. The experiment was conducted in two stages, optimization of shoot culture medium and shoot multiplication stage. In the first step, nodal cuttings are planted vertically on a solid MS medium suplemented of 1x10-5 M and 1,5x10-5 M BAP with or without NAA at concentrations of 0,5x10-6 M, 1x10-6 M, 2,5x106 M and 5x10-6 M. In the second stage is applied subculture by implanting nodal cuttings vertically and horizontally. The results of the first stage showed that the axillary shoots were highest with an average of 36,22 mm on NAA medium 2,5x10-6 M without BAP. The highest number of nodes 9,29 nodes per shoot was detected on medium with BAP 1x10-5 M without NAA, while the highest number of axillary shoots was 1,56 shoots per explant on medium BAP 1x10-5 M of combination of NAA 5x10-6 M. In the first experiment , the addition of different concentrations of BAP has an important role to increase the growth of axillary buds in terms of their effect on the increase in nodal numbers and axillary shoots number. The results of the second phase experiment showed that nodal cuttings are planted vertically had the highest shoot lengths with an average of 48 mm, the largest number of nodes 17,11 nodes per shoot and the axillary shoot rate of at most 2,78 shoots per explant. Nodal cuttings which are planted horizontally have the highest shoot length with an average of 86,11 mm, the highest number of nodes is 15,44 nodes per shoot and the axillary shoot number is at most 1,89 shoots per explant. From this research it can be concluded that nodal cuttings grown on BAP medium 1,5x10-5 M and NAA 2,5x10-6 M horizontally are more influential in inducing the length of axillary shoot and nodal number, while vertical planting produces more axillary shoots.