PRODUCTION AND CHARACTERIZATION BIOPLASTIC FROM BACTERIA STAPHYLOCOCCUS ARLETTAE BK-HRG1 WITH PALM OIL LIQUID WASTE AS A CARBON SOURCE

• Main Author: SYAADAH (NIM : 10513088), WIDA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/24691
• Institution: Institut Teknologi Bandung

Increasing production plastic can result to increasing plastic waste in the world. Plastic is non-degradable polymer. Bioplastic from PHA (Polyhydroxyalkanoat) can be one solution offered, because PHA is degradable polymer. PHA can produced intracellularly by bacterial with fermentation process. Media condition to produce PHA with abundant carbon source and O, N, S, P limited. One of carbon source is palm oil liquid waste. The purpose this study was to determination compositon palm oil liquid waste, optimum formula to produced bioplastic, and bioplastic characteristics from Staphylococcus arlettae BK-HRG1. Bioplastic production has been carried out with a source of palm oil liquid waste and a carbon source of glucose. GC-MS analysis shows that the main composition of palm oil liquid waste is glycerol. Staphylococcus arlettae BK-HRG1 (glucose and palm oil liquid waste as carbon source) has been confirmed to potentially produce PHA by grown in nile red media. Cell pellets from bacteria are dried with freeze dry, then extracted (NaOCl: chloroform). The chloroform phase is precipitated with methanol, then filtered and dried in an oven (40 °C). Optimum efficiency production bioplastic is 6,83%, which is achieved by optimum media. That is 10% palm oil liquid waste, yeast extract as nitrogen source with a concentration of 0,2%. The characterization of bioplastics (palm oil liquid waste and glucose as carbon source) by using FTIR show peak at wave number 3443 cm-1 (-OH), 2800-3000 cm-1 (CH3-CH2-), 1722 cm-1 (C = O), 1000-1300 cm-1 (C-O-C). Bioplastics (palm oil liquid waste and glucose as carbon source ) analyzed with DSC (Tm = 159,2 °C, Td = 228,8 °C and 279 °C, Xc = 33% ; Tm = 162,5 °C, Td = 221,2 °C and 290 °C, Xc = 20%).