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The prevalence of diarrhea in Indonesia is 9%. Diarrhea is a disease characterized by the loose up <br /> <br /> to three times a day marked by an increase in water content in the stool, fecal volume, and volume of <br /> <br /> fecal expenditure. Diarrhea can be caused by ba...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/24963 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | The prevalence of diarrhea in Indonesia is 9%. Diarrhea is a disease characterized by the loose up <br />
<br />
to three times a day marked by an increase in water content in the stool, fecal volume, and volume of <br />
<br />
fecal expenditure. Diarrhea can be caused by bacterial or non-bacterial infections. Some types of bacteria <br />
<br />
that can cause diarrhea were Escherichia coli, Salmonella, Staphylococcus aureus, and Pseudomonas <br />
<br />
aeruginosa This study aims to determine antimicrobial activity, minimum inhibitory concentration, and <br />
<br />
minimum bactericidal concentration ethanol extract sawo duren (Chrysophyllum cainito) leaves and barks <br />
<br />
along with its fraction against some diarrhea-causing bacteria and determine compounds that have <br />
<br />
antibacterial activity. Determination of antimicrobial activity was determined by disc diffusion and <br />
<br />
microdilution method. Determination of spots that have antimicrobial activity in chromatograms was <br />
<br />
carried out by bioautography method. The test begins with a preliminary test with disc diffusion method <br />
<br />
to determine the most susceptible test sample microbes.Based on the preliminary test, the minimum <br />
<br />
inhibitory concentration and minimum bactericidal concentration were determined by the microdilution <br />
<br />
method. Then, the spots that have antimicrobial activity were determined by bioautography method. <br />
<br />
Based on the results of preliminary tests performed, ethanol extract of sawo duren has antimicrobial <br />
<br />
activity against Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus. While ethanol <br />
<br />
extract of sawo duren bark does not have antimicrobial activity against all test microbes. Based on <br />
<br />
preliminary tests, it was found that Escherichia coli is the most sensitive microbial to the ethanol extract <br />
<br />
of sawo duren leaves. Then continued with determination of minimum inhibitory concentration and <br />
<br />
minimum bactericidal concentration of ethanol extract, water fraction, n-hexane fraction, and ethyl acetate <br />
<br />
fraction by microdilution method. The smallest MIC and MBC against E. coli were obtained by ethyl <br />
<br />
acetate and ethanol extract of sawo duren leaves were 256 µg/ml and 586 µg/ml, while MIC and MBC <br />
<br />
water fractions were 586 µg/ml and 2343 µg/ml, and n-hexane fraction were ≥ 4096 µg/ml and ≥ 18000 <br />
<br />
µg/ml. Based on bioautographic tests, it is estimated that compounds that have antibacterial activity are a <br />
<br />
class of phenol compounds and flavonoids. <br />
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