ENHANCEMENT OF LUTEIN & ZEAXANTIN CONTENT AND PRODUCTIVITY IN CHLORELLA PYRENOIDOSA USING NPK FERTILIZER

• Main Author: Resti Avrilia (Nim : 11214016), Adella
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/24967
• Institution: Institut Teknologi Bandung

Chlorella pyrenoidosa is a microalgae that is reported can synthesize lutein and zeaxanthin. This study aims to enhance lutein and zeaxanthin content and productivity using NPK fertilizer. The study was conducted by substitute the macronutrient compounds of N, P, and K in Bristol medium using NPK fertilizer.Variations of NPK fertilizer concentration used were 0,125 g/L, 0,250 g/L, 0,375 g/L, and 0,500 g/L. Cultivation was carried out for 12 days in a photobioreactor with photoperiodization 12:12, flow rate at 1.4 vvm, light intensity of 6.000 lux, pH maintained in 7–7,5, temperature 26-30˚C. Biomass of Chlorella pyrenoidosa was harvested and extracted using diethyl ether. The extract was analyzed using a spectrophotometer at λ 445 nm for lutein quantification and λ 451 nm for zeaxanthin quantification. The results of this study show that the substitution of N, P, K in Bristol medium using NPK fertilizer at concentration 0,500 g/L increasing lutein and zeaxanthin content in Chlorella pyrenoidosa. Addition of NPK produce the highest lutein content of 5,077 mg/g (140% higher than lutein in Bristol medium) and the highest zeaxanthin content of 4,133 mg/g (137% higher than zeaxanthin in Bristol medium). The maximum lutein productivity was 169 mg/L/year and maximum zeaxanthin productivity was 138 mg/L/year.