#TITLE_ALTERNATIVE#

• Main Author: IZZATUL HAQ NIM : 10714085, AHSHONAT
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/25216
• Institution: Institut Teknologi Bandung

Necrotic tissue of wound can be removed by debridement using proteolytic enzymes. This study <br /> <br /> aims to develop an in situ hydrogel preparation to create a controlled papain release. Papain was <br /> <br /> encapsulated in sodium alginate microparticles (MP). MP was prepared by mixing the sodium <br /> <br /> alginate solution and papain solution with stirring at 9000 rpm for 4 min and continued with <br /> <br /> sonication. MP formulation was optimized statistically using Box-Behnken design with factors <br /> <br /> consisting of alginate, CaCl2, and duration of sonication. The parameters used to determine the <br /> <br /> optimal MP formulas are particle size in the range 1-5 &#956;m, the maximum of entrappment efficiency <br /> <br /> (EE) and the loading of papain (LP). The optimum MP formula consisted of 0,084% alginate; 0,02% <br /> <br /> CaCl2; and 58,2 seconds of sonication with a homogenizer ultrasonic. This MP formula showed the <br /> <br /> particle size of 3,9 &#956;m; EE 82,1%; and LP 65,82%. Further, the MP was dispersed into an in situ <br /> <br /> forming hydrogel (HMP) prepared with components of alginate as the base, polyvinylohol (PVA) as <br /> <br /> humectant, and CaCl2 as crosslinking agents. The optimum HMP formula consisted of 1% alginate, <br /> <br /> 5% PVA, and 0,4% CaCl2. The HMP preparation showed a viscosity before crosslinking of 60,05+0,14 <br /> <br /> mPa and an water absorption capacity of 125+3%. Papain release of HMP was 8,62% for three days. <br /> <br /> The HMP preparation showed controlled release profile, in vitro debridement process, and in situ <br /> <br /> performance at body temperature. <br />