PROCESS ENGINEERING OF MICROBIAL XYLITOL PRODUCTION FROM OIL PALM EMPTY FRUIT BUNCH (OPEFB)

• Main Author: ASDIANA IRMA SARI YUSUF (nim : 23016002), ANDI
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/25494
• Institution: Institut Teknologi Bandung

<p align="justify">World palm oil production has opportunity continually to rise about 15.78 - 18.78 million tons up to 2025. Indonesia as the largest producer in the world will fulfill 60% of world’s palm oil need. It will release the huge solid waste especially Oil Palm Empty Fruit Bunch (OPEFB). The solid waste management in palm oil industry were still not efficient which finally caused OPEFB being incenerated or put freely in the environment. OPEFB inceneration will release CO2 in large quantities. The huge accumulation of OPEFB in the environment need very long time to be compost. Therefore, OPEFB must be utilized to prevent an increase the free accumulation and pollution of OPEFB to the environment. <br /> <br /> <br /> OPEFB can be used as raw material to produce xylitol. Xylitol from OPEFB has been explored through bioprocess routes consisting of pretreatment, enzymatic hydrolysis and xylitol fermentation. Enzymatic hydrolysis is one method often used to convert biomass lignocellulose materials into xylose (xylitol-forming monomers) because of its moderate and safe operation but its process performance still needs improvement. In this study, enzymatic hydrolysis process has been engineered by observing the effect of solid loading (5%, 10% and 15%), the addition of surfactant including Tween 80 and Tween 20 with the variations of surfactant concentration (0.5 g / L, 2.5 g / L and 5 g / L). <br /> <br /> <br /> The process engineering of hydrolysis enzymatic with these three factors showed that OPEFB hydrolysis with 15% solid loading added 5 g / L Tween 80 produced hydrolysate with the highest xylose yield around 0.0667 g / g. The best hydrolisate with the highest xylose concentration was then fermented with D.hansenii CCITB R85 for 7 days. Fermentation produced xylitol and ethanol in small amounts about 0.0011 g/g and 0.0214 g/g. Glucose and xylose were not consumed meaning that the presence of surfactant was an inhibitor in the yeast metabolic pathway to consume sugar and convert it to be xylitol.<p align="justify"> <br />