#TITLE_ALTERNATIVE#

• Main Author: UTRI AIDASARI NIM : 10714011 , ANGGI
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/25602
• Institution: Institut Teknologi Bandung

Hypertension is a risk factor for <br /> <br /> <br /> <br /> cardiovascular disease that can cause death. <br /> <br /> <br /> <br /> Traditionally, many plants have been used to <br /> <br /> <br /> <br /> lower blood pressure including binahong <br /> <br /> <br /> <br /> (Anredera cordifolia (Ten.) v. Steenis) and <br /> <br /> <br /> <br /> tempuyung (Sonchus arvensis L.) leaves. <br /> <br /> <br /> <br /> Binahong and tempuyung leaves extracts showed <br /> <br /> <br /> <br /> antihypertensive effects by inhibiting Angiotensin <br /> <br /> <br /> <br /> Converting Enzyme (ACE) and diuretics effect. <br /> <br /> <br /> <br /> The mechanism of action of binahong and <br /> <br /> <br /> <br /> tempuyung plants againts angiotensin II receptors <br /> <br /> <br /> <br /> is unknown. This study aims to determine the <br /> <br /> <br /> <br /> antihypertensive effect of ethanolic extract of <br /> <br /> <br /> <br /> binahong leaf (EEBL), ethanolic extract of <br /> <br /> <br /> <br /> tempuyung leaf (EETL), and their combination in <br /> <br /> <br /> <br /> angiotensin II-induced hypertensive animal <br /> <br /> <br /> <br /> model. The blood pressure was measured noninvasively using CODA® tail-cuff blood pressure <br /> <br /> <br /> <br /> system. The study used 27 male Wistar rats aged <br /> <br /> <br /> <br /> 4-5 months, 250-350 grams devided into nine <br /> <br /> <br /> <br /> groups; normal group, sick group, valsartan <br /> <br /> <br /> <br /> group, EEBL 50 mg/ kg bw group, EEBL 100 <br /> <br /> <br /> <br /> mg/ kg bw group, EETL 50 mg/ kg bw group, <br /> <br /> <br /> <br /> EETL 100 mg/ kg bw group, combination of <br /> <br /> <br /> <br /> EEBL –EETL (25 mg/ kg bw : 25 mg/ kg bw) <br /> <br /> <br /> <br /> group, combination of EEBL-EETL (50 mg/ kg <br /> <br /> <br /> <br /> bw : 50 mg/ kg bw) group. Rats was habituated <br /> <br /> <br /> <br /> until normal blood pressure obtained. Initial <br /> <br /> <br /> <br /> blood pressure was measured as T0, then rats <br /> <br /> <br /> <br /> were given orally: 0.5% Na-CMC solution for the <br /> <br /> <br /> <br /> normal and sick groups, EEBL 50 mg / kg bw, <br /> <br /> <br /> <br /> EEBL 100 mg / kg bw, EETL 50 mg / kg bw, <br /> <br /> <br /> <br /> EETL 100 mg / kg bw, combination EEBL-EETL <br /> <br /> <br /> <br /> (25 mg / kg bb: 25mg / kg bb), and a combination <br /> <br /> <br /> <br /> of EEBL-EETL (50 mg / kg bw: 50 mg / kg bw). <br /> <br /> <br /> <br /> One hour after given extract, angiotensin II 100 <br /> <br /> <br /> µg / kg bw diluted in 0.9% NaCl solution was <br /> <br /> <br /> <br /> injected intraperitoneally to all test groups except <br /> <br /> <br /> <br /> for normal group was injected with 0.9% NaCl <br /> <br /> <br /> <br /> solution. Two minutes after the injection, blood <br /> <br /> <br /> <br /> pressure was measured again (T1). The result <br /> <br /> <br /> <br /> shown that EEBL 50 mg/ kg bw group, EEBL <br /> <br /> <br /> <br /> 100 mg/ kg bw group, EETL 100 mg/ kg bw <br /> <br /> <br /> <br /> group, combination of EEBL –EETL (25 mg/ kg <br /> <br /> <br /> <br /> bw : 25 mg/ kg bw) group, combination of EEBL- <br /> <br /> <br /> <br /> EETL (50 mg/ kg bw : 50 mg/ kg bw) group <br /> <br /> <br /> <br /> provide prevention an increase of systolic blood <br /> <br /> <br /> <br /> pressure (SBP) and diastolic blood pressure <br /> <br /> <br /> <br /> (DBP). EEBT 100 mg / kg bw and a combination <br /> <br /> <br /> <br /> of EEBL-EETL (25 mg / kg bw : 25 mg / kg bw) <br /> <br /> <br /> <br /> can significantly prevent an increase of SBP and <br /> <br /> <br /> <br /> DBP (p <0.05) with inhibition of TDS 48.2% and <br /> <br /> <br /> <br /> 54.6%, the inhibition of TDD is 60.7% and <br /> <br /> <br /> <br /> 67.6%. Based on the results above, it can be <br /> <br /> <br /> <br /> concluded that EETL 100 mg / kg bw and <br /> <br /> <br /> <br /> combination of EEBL-EETL (25 mg / kg bw : 25 <br /> <br /> <br /> <br /> mg / kg bw can significantly reduce the action of <br /> <br /> <br /> <br /> angiotensin II (p<0.05). <br />