#TITLE_ALTERNATIVE#

• Main Author: FITRI NIM:10513022, ANNISA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/25661
• Institution: Institut Teknologi Bandung

Plants has been used by locals as traditional medicines. One of Indonesia plants that has been used for long time ago as traditional medicines is Cryptocarya genus. Cryptocarya genus belongs to Lauraceae family and it consists 327 species that distributes in Southeast Asia, Melanesia, and Australia. This genus has also been used for building material, raws material for pulps, and traditional medicines. Cryptocarya contains many secondary metabolites i.e (rumus)-pyrones, flavonoids, stilbenes, coumarins, lignans, arylpropanoid derivatives, alkaloids, terpenoids, steroids, carboxylic acid derivatives, and amide derivatives. Its major secondary metabolites are (rumus)-pyrones, flavonoids, and alkaloids. One of Cryptocarya plants which can be found in Indonesia is Cryptocarya massoy. Previous research on C. massoy has reported that the compounds isolated from C. massoy stem bark were massoialakton, n-heptyl-5,6-dyhydro-2H-pyran-2-one, goniotalamin, syringaresinol, sinapaldehyde, N-trans-feruloyltyramine, N-trans-feruloyl-3-methoxytyramine. However, the phytochemistry research on C. massoy leaves has not yet been reported. Therefore, it is important to conduct the research on C. massoy leaves to complete the phytochemistry information of this species. The isolation of secondary metabolites from C. massoy leaves have been carried out through several steps including extraction with metanol, chlorophyll separation with addition of methanol-water (1:1), partition the free chlorophyll-extract with ethyl acetate to obtain ethyl acetate extract. Then, the ethyl acetate extract was separated by Vacum Liquid Chromatography (VLC), radial chromathography (RC), and gravitational column chromathography (GCC). The structure of isolated compounds were identified by NMR spectroscopy data (1H, 13C, HSQC, and HMBC). Furthermore, the bioactivity of the extract and the isolated compounds have been examined against murine leukemia P-388 cells following MTT method. In this research, three compounds have been isolated and suggested as naringenin-7,4’-dimethylether, benzyl salicylate and benzoic acid. Naringenin-7,4’-dimethyl ether was for the first time isolated from Cryptocarya plants. The the bioactivity result against murine leukemia P-388 cells showed that the extract and also the two compounds (naringenin-7,4’-dimethylether and benzyl salicylate) were not active with IC50 value of 62.8 µg/mL, 10.91 µg/mL, >100 µg/mL, respectively.