#TITLE_ALTERNATIVE#

• Main Author: APRILLIANI NIM: 20716001, ARINI
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/25780
• Institution: Institut Teknologi Bandung

Background and purpose: Continuous ultraviolet exposure for long term will <br /> <br /> cause skin hyperpigmentation. One prevention of hyperpigmentation is inhibiting <br /> <br /> the tyrosinase activity. Some Zingiberaceae such as turmeric, galangal and <br /> <br /> bangle hantu can inhibit tyrosinase activity. This study aimed to test the inhibition <br /> <br /> of tyrosinase activity from the rhizomes of several Zingiberaceae plants. In <br /> <br /> addition, the results of selected plant fractions are also carried out to be tested. <br /> <br /> Method: Extraction by maceration is using 96% ethanol and fractionation is <br /> <br /> using n-hexane and ethyl acetate. In vitro tyrosinase inhibition test is using <br /> <br /> arbutin as positive control. Results: Test results on 19 samples show the presence <br /> <br /> of inhibition of tyrosinase activity. The ethanol extract of kecombrang, kunci <br /> <br /> pepet, bangle hitam, temu giring and red ginger give the highest inhibition <br /> <br /> against tyrosinase activity with the percentage inhibition at 22.50 ± 1.46 %, 20.75 <br /> <br /> ± 0.04 %, 19.96 ± of 0.03 %, 18.85 ± 0.11 %, and 18.63 ± 0.06 % respectively. <br /> <br /> The IC50 of kecombrang extract (761.75 ± 23.13 ppm) is higher than n-hexane <br /> <br /> (557.37 ± 34.44 ppm), ethyl acetate (542.39 ± 12.42 ppm) and water fraction <br /> <br /> (507.40 ± 2.623 ppm). So it can be concluded that the results of the three fractions <br /> <br /> have IC50 better than extract. Ethyl acetate fraction has significantly better <br /> <br /> tyrosinase inhibition than the water fraction and n-hexane fraction of kecombrang <br /> <br /> rhizome. <br />