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Diabetes Mellitus (DM) is one of the five leading causes of death in the world. The <br /> <br /> proportion of DM patients in Indonesia in 2013 is 6.9% or with an absolute number <br /> <br /> of about 12 million people. Furthermore, it is estimated that in 2030 the prevalen...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/25781 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Diabetes Mellitus (DM) is one of the five leading causes of death in the world. The <br />
<br />
proportion of DM patients in Indonesia in 2013 is 6.9% or with an absolute number <br />
<br />
of about 12 million people. Furthermore, it is estimated that in 2030 the prevalence <br />
<br />
of DM in Indonesia reaches 21.3 million people. Binahong (Anredera cordifolia <br />
<br />
(Ten.) Steenis) has traditionally been used to treat various conditions of the disease, <br />
<br />
including diabetes mellitus. Therefore, this study aims to determine the antidiabetic <br />
<br />
activity of dry leaves extract both in vivo and in vitro. The dried extract was <br />
<br />
prepared by extracting the binahong leaves with 30% ethanol, evaporated until thick <br />
<br />
and mixed with maydis starch (49%) and aerosil (1%). The antidiabetic test was <br />
<br />
performed by glucose tolerance test in rats, test in alloxan diabetic mice, liver <br />
<br />
glycogen formation, and α-glucosidase inhibitor. The dose of extract used in the <br />
<br />
glucose tolerance test was 20, 40, and 60 mg/KgBW, while the dose of extract used <br />
<br />
for the test in alloxan diabetic mice was 28, 56, and 84 mg/KgBW for 21 days. The <br />
<br />
results of oral glucose tolerance test and alloxan diabetes test showed blood glucose <br />
<br />
levels in the group given binahong extract was lower and significantly different than <br />
<br />
the diabetic group. The decrease of blood glucose level in alloxan diabetic mice at <br />
<br />
doses 28, 56, and 84 mg/KgBW was 49.58, 54.49 and 75.01%, respectively, all <br />
<br />
significantly different statistically to diabetic group. On examination of liver <br />
<br />
glycogen levels showed higher levels of glycogen in the group given binahong <br />
<br />
extract and significantly different than the diabetic group. The histologic profile of <br />
<br />
the pancreas also showed that the binahong extract was capable of repairing damage <br />
<br />
to pancreatic cells. Binahong extract was also able to inhibit the activity of α- <br />
<br />
glucosidase enzyme with IC50 value of 48.82 μg/mL. Based on these results it can <br />
<br />
be concluded that binahong extract has antidiabetic activity by repairing pancreatic <br />
β-cells, stimulates insulin release, and inhibits α-glucosidase enzyme. <br />
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