#TITLE_ALTERNATIVE#

• Main Author: HIDAYAT NIM: 21816003, ARIP
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/25788
• Institution: Institut Teknologi Bandung

Typhoid vaccine conjugate ViDT is a new vaccine and under development, <br /> <br /> therefore it is important to study critical factors that might affect its production. <br /> <br /> The aim of this study was to evaluate the effects of variations in concentration, <br /> <br /> pH, storage time and temperature on the molecular size of Vi polysaccharide, <br /> <br /> ViDT conjugate and typhoid conjugate vaccine to support the development of <br /> <br /> typhoid conjugate vaccine. Three batches of pilot scale production of Vi <br /> <br /> polysaccharide, ViDT conjugate and typhoid conjugate vaccine were tested for <br /> <br /> stability, storage temperature and pH against molecular size using the HPLC <br /> <br /> method. The particle size and microscopic of the ViDT conjugate caused by <br /> <br /> variation on the concentrations were evaluated by dynamic light scattering and <br /> <br /> scanning electron microscope, repectively. . The results of Vi polysaccharide <br /> <br /> molecule was stable in the storage for 3 months at temperature 2-8 °C, and did <br /> <br /> not affected by pH and freeze thaw. The molecular size of ViDT conjugate <br /> <br /> was stable in the storage for 3 months at 2-8 °C and 25 °C, but its molecular <br /> <br /> size was unstable in temperature of -20 °C. Change in pH did affect the <br /> <br /> molecular size of ViDT. Increasing concentrations of ViDT up to 4 times <br /> <br /> resulted an increase in molecular size and particle size, which confirmed by <br /> <br /> microscopic results. However, the increase did not statistically significant. The <br /> <br /> molecular size of typhoid conjugate vaccine was stable in the storage for 3 <br /> <br /> months at 2-8 °C and 25 °C, but there was signifficant change in molecular size <br /> <br /> at 37 °C. The molecular size of the typhoid conjugate vaccine was affected by <br /> <br /> pH. Variations in the initial concentrations of ViDT did not affect the molecular <br /> <br /> size of the typhoid conjugate vaccine. <br />