#TITLE_ALTERNATIVE#

• Main Author: NIM: 10714007, CHRISTIAN
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/26256
• Institution: Institut Teknologi Bandung

Curcumin is a phenolic compound that can be found naturally in the rhizome of Zingiberaceae <br /> <br /> plants, such as turmeric (Curcuma longa). Curcumin has wide therapeutic effects, ranging from <br /> <br /> antioxidants activity, anti-inflammatory, hepatoprotector, anti diabetic, fibrosis, and cancer <br /> <br /> treatment. However, the limitations for the development of curcumin as therapeutic agent are due <br /> <br /> to its poor solubility in water and its low bioavailability. In this study, a formulation was fabricated <br /> <br /> to overcome those limitations into a nanoemulsion dosage form using composition that was already <br /> <br /> developed in the previous study comprising castor oil as the oil phase. As surfactant and <br /> <br /> cosurfactant, cremophor RH40 and PEG 400 were used respectively. Several characterization were <br /> <br /> performed, including physical, chemical, and stability of the curcumin nanoemulsion to ensure the <br /> <br /> successful repetition of the formula. In addition, in vitro study was performed to find IC50 values <br /> <br /> and the interaction between curcumin nanoemulsion with several cell lines at several times. The <br /> <br /> observation of interaction between curcumin nanoemulsion with several cell lines was performed <br /> <br /> using confocal microscopy. The cell lines that were used in this study are cells model that represent <br /> <br /> cancerous cell of carcinoma and fibrosis. The curcumin nanoemulsion was formed spontaneously <br /> <br /> with the encapsulation efficiency of 89.022 ± 0.036 %. The obtained curcumin nanoemulsion has a <br /> <br /> droplet size of 36.83 ± 9.64 nm; polydispersity index of 0.278 ± 0.113; and zeta potential of -0.92 <br /> <br /> mV. Stability test was performed for 14 days; in room temperature. The result of the stability test <br /> <br /> indicated that curcumin nanoemulsion didn’t undergone any significant changes in its physical <br /> <br /> properties. In vitro cytotoxicity test of curcumin nanoemulsion to determine its IC50 values were <br /> <br /> performed on several cells, like 3T3/NIH, MCF-7, and MDA-MB with the MTS reagent, and were <br /> <br /> compared with the solution of curcumin in DMSO. Curcumin nanoemulsion (IC50 = 68,568±2,811 <br /> <br /> µg/mL) has a significantly lower IC50 compared to curcumin solution (IC50 = 76,981±3,457 µg/mL) <br /> <br /> on 3T3/NIH cells (p < 0.05) indicating a better cytotoxic effects. Furthermore, the cellular uptake of <br /> <br /> curcumin nanoemulsion was tested by confocal microscopy with the MCF-7 cell. DAPI dye was used <br /> <br /> to stain the nucleus while curcumin could fluorescence on its own without any labeling compound. <br /> <br /> The results showed that there was an indication of the ability of curcumin nanoemulsion and DMSO <br /> <br /> solution to penetrate the cells membrane both in incubation time of 3 and 6 hours. In conclusion, <br /> <br /> the curcumin nanoemulsion formula from previous study was successfully fabricated with satisfying <br /> <br /> results. The presence of green fluorecent at and around the cells nucleus from the observation <br /> <br /> using confocal microscope gave the initial conclusion of curcumin in the form of nanoemulsion and <br /> <br /> in the DMSO solution of having the ability to interact with the tested cell lines. <br />