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Free radicals are either atoms or molecules containing unpaired electrons in their outer orbitals <br /> <br /> so that they are very reactive. The accumulation of free radicals in the body can trigger the <br /> <br /> formation of oxidative stress which is a condition when...
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id-itb.:262732018-06-22T15:26:33Z#TITLE_ALTERNATIVE# NIM : 10714059, CHRISTOPHER Indonesia Final Project INSTITUT TEKNOLOGI BANDUNG https://digilib.itb.ac.id/gdl/view/26273 Free radicals are either atoms or molecules containing unpaired electrons in their outer orbitals <br /> <br /> so that they are very reactive. The accumulation of free radicals in the body can trigger the <br /> <br /> formation of oxidative stress which is a condition when harmful oxygen invades biological <br /> <br /> molecules such as lipids, proteins, and DNA. The human body actually has a series of defense <br /> <br /> systems against free radical attacks such as superoxide dismutase. The body must obtain intake of <br /> <br /> antioxidants from outside to prevent the occurrence of bad things that are triggered by free <br /> <br /> radicals. The widely known Annonaceae family have a very potent acetogenin compounds as a <br /> <br /> cytotoxic agent that is often used as an anticancer.This research aims to examine and compare <br /> <br /> the antioxidant activity of soursop leaves from three different areas: Bandung, Bogor, and Malang <br /> <br /> using DPPH and ABTS methods, total phenol and total flavonoid, determinethe correlation <br /> <br /> between total phenol, total flavonoid and IC50 DPPH and IC50 ABTS, determine the correlation <br /> <br /> between IC50 DPPH and IC50 ABTS on sample extract. The extraction of samples was performed by <br /> <br /> reflux method using three solvents with increasing polarity n-hexane, ethyl acetate, and ethanol. <br /> <br /> Measurements of IC50 DPPH and ABTS, total phenol and total flavonoid were performed using uvvisible spectrophotometry. The correlation between total phenol, total flavonoid and IC50 DPPH <br /> <br /> and IC50 ABTS as well as the correlation between IC50 DPPH and IC50 ABTS were done statistically <br /> <br /> by Pearson correlation method. Antioxidant activity of all extracts from three different site in the <br /> <br /> range of 0.219 􀍴34.551 µg/mL by DPPH method and in the range of 0.199 􀍴6.251 µg/mL by ABTS <br /> <br /> method. Ğä¨ äçÚä¨ĚĤĤćĤΝ ēä¨Āćù ŋŒĚçĀĤä¨ 􀁄ŒùŒĀÚ􀍛ĚĚćĿĖĚćēù¨Œves ethyl acetate extract <br /> <br /> (24.270 ± 0.438 g G!􀀜􀍬􀏭􀏬􀏬Ú􀍿􀍘Ğä¨ äçÚä¨ĚĤĤćĤΝ ²ùŒŊćĀćç¤Ěŋ¨Ė¨ çĀĤä¨ 􀀑ŒĀ¤ĿĀÚ􀍛̨Ĥäőù Œš¨ĤŒĤ¨ <br /> <br /> extract of soursop leaves (11.711 ± 1.132 g QE/100 g). Total phenol in soursop leaves extract from <br /> <br /> Bandung had significant and negative correlation with IC50 DPPH (r =-0.648, p<0.05) and IC50 ABTS <br /> <br /> (r =-0.851, p<0.01). All soursop leaves extracts from Bandung, Bogor, dan Malang were very <br /> <br /> strong antioxidant. Phenolic compounds have big contribution in antioxidant activity of soursop <br /> <br /> leaves extract from Bandung by DPPH and ABTS methods. DPPH and ABTS methods gave linear <br /> <br /> results in antioxidant activities of soursop leaves extracts from Bandung and Bogor. <br /> text |
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Indonesia |
| description |
Free radicals are either atoms or molecules containing unpaired electrons in their outer orbitals <br />
<br />
so that they are very reactive. The accumulation of free radicals in the body can trigger the <br />
<br />
formation of oxidative stress which is a condition when harmful oxygen invades biological <br />
<br />
molecules such as lipids, proteins, and DNA. The human body actually has a series of defense <br />
<br />
systems against free radical attacks such as superoxide dismutase. The body must obtain intake of <br />
<br />
antioxidants from outside to prevent the occurrence of bad things that are triggered by free <br />
<br />
radicals. The widely known Annonaceae family have a very potent acetogenin compounds as a <br />
<br />
cytotoxic agent that is often used as an anticancer.This research aims to examine and compare <br />
<br />
the antioxidant activity of soursop leaves from three different areas: Bandung, Bogor, and Malang <br />
<br />
using DPPH and ABTS methods, total phenol and total flavonoid, determinethe correlation <br />
<br />
between total phenol, total flavonoid and IC50 DPPH and IC50 ABTS, determine the correlation <br />
<br />
between IC50 DPPH and IC50 ABTS on sample extract. The extraction of samples was performed by <br />
<br />
reflux method using three solvents with increasing polarity n-hexane, ethyl acetate, and ethanol. <br />
<br />
Measurements of IC50 DPPH and ABTS, total phenol and total flavonoid were performed using uvvisible spectrophotometry. The correlation between total phenol, total flavonoid and IC50 DPPH <br />
<br />
and IC50 ABTS as well as the correlation between IC50 DPPH and IC50 ABTS were done statistically <br />
<br />
by Pearson correlation method. Antioxidant activity of all extracts from three different site in the <br />
<br />
range of 0.219 􀍴34.551 µg/mL by DPPH method and in the range of 0.199 􀍴6.251 µg/mL by ABTS <br />
<br />
method. Ğä¨ äçÚä¨ĚĤĤćĤΝ ēä¨Āćù ŋŒĚçĀĤä¨ 􀁄ŒùŒĀÚ􀍛ĚĚćĿĖĚćēù¨Œves ethyl acetate extract <br />
<br />
(24.270 ± 0.438 g G!􀀜􀍬􀏭􀏬􀏬Ú􀍿􀍘Ğä¨ äçÚä¨ĚĤĤćĤΝ ²ùŒŊćĀćç¤Ěŋ¨Ė¨ çĀĤä¨ 􀀑ŒĀ¤ĿĀÚ􀍛̨Ĥäőù Œš¨ĤŒĤ¨ <br />
<br />
extract of soursop leaves (11.711 ± 1.132 g QE/100 g). Total phenol in soursop leaves extract from <br />
<br />
Bandung had significant and negative correlation with IC50 DPPH (r =-0.648, p<0.05) and IC50 ABTS <br />
<br />
(r =-0.851, p<0.01). All soursop leaves extracts from Bandung, Bogor, dan Malang were very <br />
<br />
strong antioxidant. Phenolic compounds have big contribution in antioxidant activity of soursop <br />
<br />
leaves extract from Bandung by DPPH and ABTS methods. DPPH and ABTS methods gave linear <br />
<br />
results in antioxidant activities of soursop leaves extracts from Bandung and Bogor. <br />
|
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NIM : 10714059, CHRISTOPHER |
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NIM : 10714059, CHRISTOPHER #TITLE_ALTERNATIVE# |
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NIM : 10714059, CHRISTOPHER |
| author_sort |
NIM : 10714059, CHRISTOPHER |
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#TITLE_ALTERNATIVE# |
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#TITLE_ALTERNATIVE# |
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#TITLE_ALTERNATIVE# |
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#TITLE_ALTERNATIVE# |
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#TITLE_ALTERNATIVE# |
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| url |
https://digilib.itb.ac.id/gdl/view/26273 |
| _version_ |
1822020960649216000 |