NEXT GENERATION SEQUENCING (NGS) APPROACH FOR MONITORING THE DYNAMICS OF BACTERIAL COMMUNITIES IN BIOSTIMULATION OF OIL RESERVOIR Y, SUMATERA
<p align="justify">Microbial Enhanced Oil Recovery (MEOR) is a technique to optimize oil recovery by using microbial activity. The monitoring of the bacterial community is done after the injection of nutrients consisting of molasses (6.54%), NPK (0.25%) and DAP (0.31%). This monitori...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/26279 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | <p align="justify">Microbial Enhanced Oil Recovery (MEOR) is a technique to optimize oil recovery by using microbial activity. The monitoring of the bacterial community is done after the injection of nutrients consisting of molasses (6.54%), NPK (0.25%) and DAP (0.31%). This monitoring is necessary to obtain information on indigenous bacteria as well as monitor the growth of harmful microbes. The purpose of this study was to determine the effect of nutritional injections on the dynamics of the bacterial community, to determine the predominant bacteria, and to determine the relationship of physicochemical characteristics to the indigenous bacteria community Y during the monitoring period. The study was conducted by extracting DNA from oil brine samples before and after injection. The microbial community dynamics analysis was performed with Illumina Hisses 2500 using the 16S rRNA region V3-V4 gene and performed bioinformatic analysis using QIIME and R studio. Saturated, Aromatic, Resin, Asphaltenes (SARA) saturated hydrocarbon fractions at the five monitoring time points were analyzed using column chromatography. The results showed that 39 genera were detected from eight Bacterial phyla and one phylum of Archaea. Sample before the injection is predominated by communities of hydrocarbonclastics. A predominant group of molase bacterial users was found in the sample of second weeks after injection. Meanwhile, the sample of fourth week after the injection began to show predominance toward hydrocarbonoclastic bacteria and surfactant-producing bacteria. The sixth and eighth week samples showed predominations of hydrocarbonoclastic bacteria. The decrease in pH value is found to be along with the presence of acid-producing bacterial groups, while the decreasing viscosity is coincidence with the emergence of groups of bacteria capable of hydrocarbon degradation, and IFT (interfacial tension) decrease when a groups of surfactant-producing bacteria is found in the community.<p align="justify"> |
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