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<p align="justify"> <br /> <br /> <br /> Vibriosis syndrome is the main issue encountered by shrimp industry globally. Vibriosis is caused by pathogenic bacteria Vibrio sp. and significantly affect the shrimp survival rate. Since the usage of antibiotics is prohibi...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/26394 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | <p align="justify"> <br />
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Vibriosis syndrome is the main issue encountered by shrimp industry globally. Vibriosis is caused by pathogenic bacteria Vibrio sp. and significantly affect the shrimp survival rate. Since the usage of antibiotics is prohibited in aquaculture industry, new approach is necessary through microbial interaction manipulation which will disturb Quorum Sensing system due to the disruption of autoinducer Vibrio sp., N-Acyl-Homoserine-Lactone (AHL). This research aims to observe the effect of bacteria consortium addition Nitratireductor aquibiodomus and Halomonas aquamarina on virulence reduction of Vibrio sp. toward nursery PL-5 udang putih (Litopenaeus vannamei) and to determine the survival rate of white shrimp larvae after the challenge test with Vibrio sp. . This research is divided into three consecutive stages : (1) Antagonistic test between Halomonas aquamarina and Nitratireductor aquibiodomus (2) quantification of bacteria consortium ability in disrupting Vibrio sp. Quorum Sensing system and (3) white shrimp post-larvae challenge test against Vibrio sp. with 6 different treatments during 5-day culture period. The result showed that bacteria consortium can reduce bioluminescence for 51.38%. Addition of bacteria consortium to the white shrimp culture significiantly improved survival of the post-larvae at three different delivery time (p<0.05) when challenged with Vibrio sp. Halomonas aquamarina and Nitratireductor aquibiodomus addition 24 hours before Vibrio sp. addition gave the highest survival of white shrimp post-larvae 0f 65.53%, followed by simultaneous addition of bacteria consortium and Vibrio sp. (survival : 33%) and bacteria consortium addition 24 hours after addition of Vibrio sp. (survival : 32.07 %). All of those treatments was significiantly different compared to single addition of Vibrio sp. (survival : 21%). It could be concluded that Halomonas aquamarina and Nitratireductor aquibiodomus are able to reduce Vibrio sp. and significantly increase the survival of white shrimp post-larvae with best challenge test result obtained in the culture with the addition of Halomonas aquamarina and Nitratireductor aquibiodomus 24 hours before addition of Vibrio sp. <br />
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