BACTERIAL GROWTH KINETIC ON BIOREMEDIATION OF SOIL CONTAMINATED OF TEXTILE EFFLUENT BY FORCED-AERATED STATIC PILE TECHNIQUE (CASE STUDY: AGRICULTURAL FIELD RANCAEKEK AREA)

• Main Author: PRIMANANDI (NIM: 25315013), DENDY
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/26478
• Institution: Institut Teknologi Bandung

The development of the textile industry both small and large scale is increasing. Various kinds of pollutants in textile waste products, especially dyes, can endanger both the surrounding environment and human health. The use of microorganisms such as bacteria as a technology with biological principles can be enhanced by efforts to maximize the ability of bacteria to degrade or decolorize waste colors produced from textile industry waste. The selection of sampling locations for textile waste contaminated soil in this study was at the location of agricultural land in the area around the Rancaekek textile industry, West Java. The condition of agricultural land looks very black. <br /> <br /> <br /> The ability of indigenous bacteria to decolorize textile color waste was tested by isolating the bacteria to be used as isolates in the Salt Based Solution (SBS) liquid minimum media. The next process is to determine some biodecolorization kinetics parameters. As for the calculation of several kinetics parameters which are carried out respectively for the specific growth rate (&#956;), the maximum specific growth rate (&#956;max), the half saturation constant (KS), the efficiency of cell synthesis (Y), the specific substrate utilization rate (q), the maximum specific substrate utilization rate (qmax), and the endegenous mortality coefficient (kd) is equal to 0,1111-0,1326/hour; 0.1979/day; 189 TCU mg/l; 0,0028; 39,7-47,4/hour;70,69/hour; and 0,0033/hour. <br /> <br /> <br /> The bacterial consortium that has been obtained from the previous isolation results was used in the laboratory-scale decolorization test with the aim of the success of the biodegradation process to be carried out. Test results of the initial characteristics of both physics, chemistry and biology were carried out on soil samples. Soil type is classified as humus clay type with a porosity level of 83%. The measured initial color concentration was 1,846 TCU mg/l and the bacteria obtained at the beginning were for heterotrophs 210.104 CFU/g soil and for autotrophs 217.103 CFU/g soil. Variations in air rate and pH were tested for biodegradation running on a simple reactor scale with a batch system with the final results for variations in air rate 0,6; 1,05; 1,35; 1,8 LPM, and accompanied by control without adding any air injection, respectively 1.043 TCU mg/l, 941 TCU mg/l, 1.144 TCU mg/l, 1,364 TCU mg/l, and 1.337 TCU mg/l. The highest population dynamics of autotrophic bacteria at optimum flowrate (1,05 LPM) were 72,5.105 CFU/g soil (74th-day). For decolorization with a variation of pH of 4-5, 6-7, 7-8, 8-9, and accompanied by a pH control of 5-6 consecutively is 1.241 TCU mg/l, 1.018 TCU mg/l, 783 TCU mg/l, 881 TCU mg/l, and 1.326 TCU mg/l. The highest bacterial population dynamics at optimum pH (7-8) are as much as 286.107 CFU/g soil (49th-day). The highest efficiency at the flowrate and optimum pH were 49% and 58% respectively. Further studies are needed regarding the increasing success of the decolorization efficiency of textile color waste bioremediation with a case study on one of the Rancaekek's agricultural lands and its application on a field scale. <br />