ISOLATION, SELECTION, CHARACTERIZATION OF BIOSURFACTANT PRODUCING BACTERIA AND ITS APPLICATION ON BIODEGRADATION OF PETROLEUM HYDROCARBON

• Main Author: MELATI ARSYAH, DENISA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/26482
• Institution: Institut Teknologi Bandung

Biosurfactants are amphiphilic compounds produced by microorganisms and possess surface-active property, hence permitting them to grow on or take up hydrophobic substrates. With its physical property, biosurfactants have been studied for its environmental applications. In this research, several petrophilic bacteria are undergo selection process to obtain bacteria isolates that capable of producing biosurfactants. The presence of biosurfactants is measured by emulsification index (%EI24) and surface tension. From the selection process, two different kinds of bacteria are isolated, which are Pseudomonas aeruginosa PAU01 and Burkholderia sp. PAU02, with biosurfactant produced by Pseudomonas aeruginosa PAU01 measured with surface tension of 31.8 dyne/cm, about 56% reduction of water surface tension of 72 dyne/cm; and 79% of emulsification index, while biosurfactan from Burkholderia sp. PAU02 altering the reduction of surface tension to 44.5 dyne/cm and emulsification index of 84%. Biosurfactants yield are also studied, both bacteria yielded maximum amount of biosurfactants on stationary phase, after 36-hour incubation period, resulting 6.98 g/l and after 42-hour incubation, resulting 5.18 g/l for biosurfactants produced by Pseudomonas aeruginosa PAU01 and Burkholderia sp. PAU02, respectively. This is indicating that biosurfactants produced by both bacteria are secondary metabolite. CMC measurement revealed concentration of 134 mg/L and 279 mg/L, respectively. It was proved that with addition of biosurfactants produced by Pseudomonas aeruginosa PAU01 with 1,01×CMC amount could enhance the rate of biodegradation efficiency in removing petroleum hydrocarbon up to 66,03% and 65,3% of removal efficiency with addition of biosurfactant from Burkholderia sp. PAU02 with 0,34×CMC amount after 10-days incubation period. These finding further indicate that biosurfactants produced by the isolates could be useful for bioremediation application.