#TITLE_ALTERNATIVE#

• Main Author: FAISHAL KUSINDARTO NIM : 10714053, DENOBIA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/26496
• Institution: Institut Teknologi Bandung

Green tea (Camellia sinensis (L.) Kuntze) leaves have been known empirically and scientifically to <br /> <br /> reduce dental plaque formation caused by Streptococcus mutans. The purpose of this research was <br /> <br /> to develop sugar-free soft candy product formula containing green tea leaves extract for dental <br /> <br /> health care and in vitro dental antiplaque activity testing against Streptococcus mutans. Green tea <br /> <br /> leaves extract was prepared by extraction with decoction method, which is heating dried green tea <br /> <br /> leaves powder in boiling distilled water, then concentrated. The preparation of soft candy included <br /> <br /> ingredients mixing, heating, molding, and cooling process. The final product were tested for in vitro <br /> <br /> dental antiplaque activity in the form of inhibition of biofilm formation against Streptococcus <br /> <br /> mutans with microtiter plate method which is quantified using crystal violet. Sugar-free soft candy <br /> <br /> final formula consisted of 5% green tea leaves extract, 12.5% gelatine, 20% sorbitol, 30% xylitol, <br /> <br /> 0.1% sodium metabisulphite, 0.1% potassium sorbate, 0.77% of 10% v/v peppermint oil in <br /> <br /> propylene glycol, and water until 100% w/w. Small pieces of products approximately 1.4 grams that <br /> <br /> have been sonicated in sterile distilled water (1:1) at a frequency of 59 kHz for 1 minute showed <br /> <br /> percentage of inhibition of biofilm formation of 82.40 ± 4.98%. It was statistically significantly <br /> <br /> different from blank and not significantly different from L commercial mouthwash containing green <br /> <br /> tea extract using independent t-test at &#945;= 0.05. This sugar-free soft candy product containing green <br /> <br /> tea leaves extract have the potential to be further developed as an alternative preparation for <br /> <br /> dental health care. <br />