SYNTHESIS OF FE(III)-SALEN AS A SUPEROXIDE DISMUTASE MIMIC

• Main Author: TUTI ADI NINGSIH NIM:10512072, DIEGOS
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/26647
• Institution: Institut Teknologi Bandung

Metal complexes formed by transition metals and Schiff base ligands are best known for their applications in biomedic, biomimetic, and catalytic system. In this research, the potential of Fe(III)-salen complex as SOD mimetics was studied. Superoxide dismutase is a metalloenzyme that converts superoxide radicals (O2.(rumus)) to hydrogen peroxide (H2O2) and oxygen (O2). Salen compound has been synthesized from salicylaldehyde and ethylenediamine (2:1). It has a melting point of 121-122oC. Infrared spectrum of salen compound showed vibration from C=N bond at 1637 cm-1 which is the special characteristic of a Schiff base ligand. The 1H NMR spectrum of salen compound gave signals at the chemical shifts of 6,82-7,26 ppm and 8,35 ppm which are originated from H atom of aromatic and HC=N groups. Fe(III)-salen complex was synthesized from Fe(NO3)3.9H2O and salen with 1:1 ratio. The mass spectrum of the complex showed that its molecular weight was 323,16 g/mole which matched with the molecular formula of [Fe(C16H14N2O2]+. Its UV/Vis spectrum showed maximum absorbancy at 260 nm and 335 nm which indicated the d-d’ transition. Fe(III)-salen is a paramagnetic compound with the ?eff of 5,66 BM. The SOD bioactive assay using non-enzymatic NBT/riboflavine method showed IC50 value at 2,98 ?M. Therefore, Fe(III)-salen have the potential as SOD mimetics.