THE CONTENT OF HEAVY METAL ELEMENT AND VITAMIN E ON WISTAR RAT TESTIS AFTER BEING EXPOSED TO TPAs SARIMUKTI 'S LEACHATE

• Main Author: NURMA RIANA - NIM: 20615011 , ELISA
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/26911
• Institution: Institut Teknologi Bandung

<p align="justify">TPA Sarimukti leachates has proved to cause testicular abnormalities, morphological abnormalities of spermatozoa, and spermatozoa DNA damage. One of the suspected causes is chemical compounds which present in leachates. A type of compound which is exist in leachate and have genotoxic and reproduction abnormalities properties is heavy metal elements. This element can cause disruption of the testes tissue structure and the process of spermatogenesis, resulting on the abnormality of spermtozoa. Heavy metals can cause such damage allegedly due to an antioxidant imbalance in the testis. To prove the testicular damage, spermatogenesis disorder, and antioxidant imbalance in testes caused by TPA Sarimukti leachate, this study aims to determine the content of heavy metals in testes of male Wistar rats exposed to TPAs Sarimukti leachate, as well as to know the concentration of vitamin E testis Wistar rat exposed to TPAs Sarimukti leachate. Twenty four male Wistar rats (age 10 weeks, weight 225-275 grams) were divided into four groups: (a) control group (ddH2O) (b) 63% leachate (c) CdCl2 (d) H2O2. The administration was done intraperitoneally (IP) within the duration of 14 days. On the 15th day, the rats were dissected and the testis was isolated. The concentration of heavy metals was measured by using the Atomic Absorption Spectrometry (AAS) method, while vitamin E consentration was measured by using the High Performance Liquid Chromatography (HPLC). The results showed that the concentration of Zn inside the testicular tissue is 0.6 ppm when compared to control of 0.5 ppm, while other metals concentration are 0 ppm. This may due to several factors, one of the six heavy metals in the low leachate air is likely low. The scope is excellent in various types, and others in the landfill, waste origin, and sampling time (season). A very heavy concentration on the testes and the effects of liver biotransformation. Metal weight is not found in the testes. However, this has a conflicting effect between bioactivation and detoxification on the biotransformation process in the liver, thus forming an <br /> <br /> excessive intermediate reaction. The results of this revolution that can cause increased ROS in the liver. The result of measurement of vitamin E in air skin 63% is very low that is 1.06 ppm compared to control of 7.92 ppm and higher than control treatment of CdCl2 and H2O2 0.53 ppm and 0.85 ppm respectively. This suggests that Sarimukti TPA water can produce ROS as indicated by biomarker decrease in Vitamin E concentration in testes causing cell membrane damage with resistance and testes.<p align="justify">