#TITLE_ALTERNATIVE#

• Main Author: KHOIRUNNISA MAESAROH NIM : 10714013, ELYA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/26928
• Institution: Institut Teknologi Bandung

Peroral routes drug delivery is a preferable drug delivery system in order to provides convenience <br /> <br /> and ease of use for patients than other delivery routes. However, peroral route provides problems <br /> <br /> such as level of acidity or alkalinity of gastrointestinal tract that may affect the properties of drug <br /> <br /> compound and some drug compounds have poor solubility and permeability. It affects the drug <br /> <br /> absorption and permeability. Conjugation formation of drug and targeting protein like lectin is a <br /> <br /> method to overcome the problems. Lectin can recognize and bind to specific sugars in <br /> <br /> gastrointestinal tract to be absorb. Formation of bioconjugation can be formed with Light Subunit <br /> <br /> Mushroom Tyrosinase (LSMT) is light chain of tyrosinase enzyme from Agaricus bisporus that has <br /> <br /> the ability to recognize specific sugars, non-toxic and non-immunogenic. The aim of this study is to <br /> <br /> determine the formation of biocojugation of captopril with LSMT and its characteristics. Formation <br /> <br /> of bioconjugate uses N-hydroxysuccinimide (NHS) linker to activate captopril on the carboxylic <br /> <br /> group then reacted with LSMT at room temperature in 4 hours with ratio of captopril:NHS:protein <br /> <br /> was 100:10:1 and resulted yield in first reaction was 83.00% and second reaction was 84.59%. The <br /> <br /> formed bioconjugates were characterized by Thin Layer Chromatography (TLC), UV-Vis <br /> <br /> Spectrophotometry, High Performance Liquid Chromatography (HPLC) and Fourier Transform Infra <br /> <br /> Red (FTIR) Spectrometry. Based on that characterized results, it is concluded that captopril can be <br /> <br /> conjugated with LSMT. <br />