#TITLE_ALTERNATIVE#

• Main Author: NURANI REZEKI NIM : 11614016, EVANDA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/27010
• Institution: Institut Teknologi Bandung

Free radicals are atoms or group of atoms or molecules that have groups of unpaired electrons. <br /> <br /> The existence of unpaired electrons in the outer orbital of a free radical, causing that radical attack <br /> <br /> molecules on its surrounding areas to achieve stability. These reactive compounds cause <br /> <br /> degenerative diseases include cancer. These negative impacts can be reduced by antioxidants. <br /> <br /> Types of plants included arumanis mango are reported to have antioxidant capacity. Antioxidants <br /> <br /> are compounds that can inhibit the oxidation reaction to scavenge free radicals. The objectives of <br /> <br /> this research were to study antioxidant activity of peel, flesh, and seed of arumanis mango by using <br /> <br /> DPPH (2,2-diphenyl-1-picrylhydrazyl) and CUPRAC (Cupric Reducing Antioxidant Capacity) <br /> <br /> methods; total flavonoid and phenolic content; analyze the correlation between total flavonoid <br /> <br /> and phenolic with their IC50 DPPH and EC50 CUPRAC, and analyze the correlation beetwen DPPH <br /> <br /> and CUPRAC in sample extracts. Samples were extracted by reflux apparatus using increasing <br /> <br /> polarity of solvent. Determination of IC50 of DPPH, EC50 of CUPRAC, total flavonoid and total <br /> <br /> phenolic content of each extract were performed by UV-visible spectrophotometry and correlation <br /> <br /> of total flavonoid and phenolic content with IC50 of DPPH and EC50 of CUPRAC activities were <br /> <br /> analyzed by Pearson’s method. Ethanolic seed extract of arumanis mango had the lowest IC50 of <br /> <br /> DPPH (4,09 µg/mL) and ethyl acetate flesh extract of arumanis mango had the lowest EC50 of <br /> <br /> CUPRAC (18.56 µg/mL). Ethanolic peel extract of arumanis mango had the highest flavonoid <br /> <br /> content (8.61 g QE/100 g) and ethyl acetate seed extract of arumanis mango showed the highest <br /> <br /> total phenolic content (22.45 g GAE/100 g). Total flavonoid content in extract seed of arumanis <br /> <br /> mango had negative and significant correlation with their IC50 of DPPH. Total phenolic content in <br /> <br /> extract flesh of arumanis mango had negative and significant correlation with their IC50 of DPPH. <br /> <br /> Flavonoid compounds were the major contributor in antioxidant activity of seed extract of <br /> <br /> arumanis mango with DPPH method. Phenolic compounds were the major contributor in <br /> <br /> antioxidant activity of flesh extract of arumanis mango with DPPH method. DPPH and CUPRAC <br /> <br /> methods gave linear result in antioxidant activity of seed extract of arumanis mango. <br />