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Indonesia belongs to largest Moslem country in the world with the Moslem <br /> <br /> population of around 89%. Consequently, Islamic way of life, including concept of <br /> <br /> halal, is practized in daily life in Indonesia. However, currently there are many cases <b...
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| Main Author: | |
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/27232 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Indonesia belongs to largest Moslem country in the world with the Moslem <br />
<br />
population of around 89%. Consequently, Islamic way of life, including concept of <br />
<br />
halal, is practized in daily life in Indonesia. However, currently there are many cases <br />
<br />
of pork mixing in processed products circulating in the community because the <br />
<br />
price of pork is relatively cheaper than beef. Therefore, in this research, an <br />
<br />
analytical method development based on specific meat protein contained different <br />
<br />
animal species, including chicken, beef, lamb and pork was conducted. The specific <br />
<br />
meat protein of pork is especially important for pork identification in processed <br />
<br />
food product. The meat protein isolation was conducted by centrifugation followed <br />
<br />
by precipitation using ammonium sulfate with different saturation level and dialysis <br />
<br />
using Phosphate Buffered Saline (PBS). Proteins were identified by means of <br />
<br />
Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) after <br />
<br />
purification using ion exchange chromatography and gel filtration chromatography. <br />
<br />
The isolated specific protein was quantified using the Lowry method and then <br />
<br />
immunized into the BALB/c strain mice. The polyclonal antibodies formed were <br />
<br />
tested using antibody titres and agglutination tests. The results showed that the <br />
<br />
differenciating specific protein of pork from chicken meat, cow, and goat was found <br />
<br />
to have molecular weight of 19,736 KDa as could be observed in electrophoregram. <br />
<br />
The agglutination test results revealed that, the reaction between the specific <br />
<br />
polyclonal antibodies of pork with the crude antigen protein of pork resulted in <br />
<br />
agglutination, while in the case of chicken, beef and lamb no agglutination was <br />
<br />
observed. <br />
<br />
Keywords: Meat, specific protein, polyclonal antibody <br />
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