Architecture And Permeability Evaluation Of Silk Fibroin Scaffold from Direct Dissolution Salt Leaching Method

• Main Author: RAMDANI NUGRAHA (NIM : 13713501), FEBRI
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/27260
• Institution: Institut Teknologi Bandung

<p align="justify"> Tissue engineering scaffold is used as a template to facilitate and support the attachment, growth, proliferation, and differentiation of cells or tissues. The fabricating of silk fibroin scaffold with the direct dissolution salt leaching method has been successfully developed to produce biocompatible and biodegradable scaffolds that support cell growth, and significantly reduce processing time and temperature compared to conventional methods. The scaffold architecture has a direct impact on attachment, growth, and migration of cells through the scaffold. Permeability affects the diffusion of nutrients, oxygen, waste, and cell migration into the scaffold. In this study evaluation of pore architecture and permeability of scaffolds, which is the result direct dissolution salt leaching method. The evaluated scaffold architecture are pore size, scaffold porosity, wall thickness scaffold, pore interconnectivity, and the scaffold specific surface area. The varied process parameters are NaCl particle size (158 ± 47 &#956;m, 250 ± 58 &#956;m, 378 ± 42 &#956;m, 503 ± 31 &#956;m), and silk fibroin concentration (6% w / v, 8% w / v, 10% w / v, 12% w / v). The evaluation of scaffold architecture using microcomputed tomography (micro-CT) method. The scaffold permeability measured using falling head method. The 3D evaluation of micro-CT can be used to evaluate pore size, scaffold porosity, wall thickness scaffold, pore interconnectivity, and the scaffold specific surface area. The increasing of NaCl particle size generates increasing of pore size, scaffold porosity, scaffold wall thickness, pore interconnectivity, and the scaffold specific permeability, while the scaffold specific surface area was decrease. The increasing of silk fibroin concentration doesn’t result in significant changes in scaffold architecture and scaffold permeability. The larger of pore size, the larger of scaffold porosity, wall thickness scaffold, pore interconnectivity was result, but the smaller of scaffold spesific surface area. The higher of the scaffold porosity, and the scaffold pore interceptivity, while the lower scaffold specific surface area, results in higher specific scaffold permeability. <br /> <p align="justify">