PRODUCTION, CHARACTERIZATION AND APPLICATION OF LEVAN FROM Bacillus licheniformis BK2 FOR BIONANOPARTICLE LEARNING MEDIA

• Main Author: FARIDATUL HABIBAH (NIM:90516007), FERA
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/27290
• Institution: Institut Teknologi Bandung

Typical properties of bionanoparticles are biodegradable, biocompatible and non alergic. <br /> <br /> Therefore, they are recently applied in some industrial fields, for example as enzymes <br /> <br /> immobilizer. The immobilized enzymes have better stability against variation in pH, <br /> <br /> temperatures and have better reusability. It is because bionanoparticles can provide <br /> <br /> structural protections from direct physical and chemical changes in the environment. In <br /> <br /> addition, bionanoparticles are relatively easy to be prepared. Therefore, the objective of <br /> <br /> this study is to provide learning media for undergraduate student about how to prepare <br /> <br /> bionanoparticles for enzymes immobilization. The biopolymers that was used in this <br /> <br /> study is levan, a fructo-oligosaccharide or also known as fructan. In this study, levanbased <br /> <br /> nanoparticles were used to immobilize Lsbl-bk2 recombinant levansucrase from <br /> <br /> Bacillus licheniformis BK2 and lipase from porcine pancreas. Levan was produced in <br /> <br /> vitro by using recombinant levansucrase from Bacillus licheniformis BK2 as biocatalyst. <br /> <br /> Levan produced by this procedure was characterized its structure by FTIR. <br /> <br /> Bionanoparticles for enzymes immobilization were prepared by stirring method. Three <br /> <br /> stirring speeds, which were 200, 500, 700 rpms, were used in bionanoparticles preparation <br /> <br /> to study its effect towards nanoparticles-size distribution and enzyme activity. The <br /> <br /> immobilization efficiency of enzymes was calculated based on the percentage of bound <br /> <br /> enzymes in the bionanoparticles. Morphology of the bionanoparticles was analysed by <br /> <br /> scanning eletron microscopy (SEM), while their size distribution was evaluated by <br /> <br /> particle size analyser (PSA). In addition, the efficiency of enzyme encapsulation by <br /> <br /> bionanoparticles was measured by zeta potential. The result of analysis showed that both <br /> <br /> levansucrase and lipase were better immobilized in levan-based nanoparticle by using <br /> <br /> 500 rpm of stirring speed. The bionanoparticles were more homogenous in particles size <br /> <br /> and can improve enzyme activity. Based on this result, learning media was made in the <br /> <br /> form of demonstration videos and experimental protocol. The assessment to the learning <br /> <br /> media by polling student’s opinion showed that most students were easily followed the <br /> <br /> instruction and both learning media can be used for self learning material for <br /> <br /> bionanoparticles-based enzyme immobilization.