ELECTROPOLYMERIZATION OF MOLECULARLY IMPRINTED POLY(METHYL ORANGE) ON SURFACE OF CARBON PASTE ELECTRODE FOR VOLTAMMETRIC DETERMINATION OF BHA AND CAP

• Main Author: FADILLAH (NIM : 20516019), GANJAR
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/27454
• Institution: Institut Teknologi Bandung

Butyl Hidroksi Anisol (BHA) and chloramphenicol (CAP) are additive compounds which found in comercial product. In Indonesia BHA is mostly found in easily oxidized foods such as vegetable oil and margarine while CAP is widely found as a residue on animal prodak such as milk. However, the use of both compounds may have a carcinogenic effect and even produce toxic substances to human life. Therefore, considering the importance of the analysis of both compounds and the regulation are strict limited by legislation. So in this study the modified carbon paste electrode (CPE) with Molecularly Imprinted Polymers (MIPs) for the determination of BHA and CAP has been developed. The modification and deposition of MIPs on the surface of CPE was assembled by electropolimerization process using Cyclic Voltammetry (CV) technique in BHA/CAP solution as template molecule and methyl orange (MO) as functional monomer with phosphate buffer as supporting electrolyte. Composition of template and monomer, pH of analyte solution, electropolimerization cycles, removing of molecules tempalte from matrix polymer and interferents study have been optimized. Analytical measurement results using Differential Pulse Voltammetry (DPV) show that modification can improve the current response and sensitivity of the electrode. The calibration curve provides that a linear range of sensor was from 10 to 400 μM for BHA and 10 to 600 μM for CAP with the limit of detection (LOD) of 0.94 μM and 3.90 μM (3σ), respectively. The proposed sensor displayed high selectivity, excelent stability and good reproducibility (n=40) for determinantion of BHA and CAP. The results of determination in real samples with the modified electrode MIPs were in good agreement with those HPLC method with agreement at 95 % confidence level.