Biosurfactant Potential of Indigenous Bacteria Consortium from Oilfield Reservoir in MEOR Implementation

• Main Author: Suhairi - Nim: 10613038, Hafidzurrahman
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/27634
• Institution: Institut Teknologi Bandung

<p align="justify">Enhancement of oil reserve should be improved through several alternatives, among them considered as Microbial Enhanced Oil Recovery (MEOR) with prosperous of economical, environment sustainability, and metabolite/bioproduct producible. Biosurfactant conducts as decreasing of oil-water interfacial tension, emulsifier as well as oil’s wettability through rock’s capillary porous of reservoir. Therefore, the research aims to determine the optimization of selected nitrogen source and their concentration variation based on growth as well as biosurfactant production of indigenous bacteria consortium from Bacillus subtilis BH 1.3 & SM 2.3. Urea, NPK, and ammonium nitrate were selected as nitrogen source’s variable test. The optimization of concentration variation among optimal’s nitrogen source are 1,0 g/L (w/v), 3,0 g/L (w/v), and 4,0 g/L (w/v). The growth’s curve and biosurfactant production’s curve as also Critical Micelle Concentration (CMC) were established. The type of biosurfactant which produced by consortium was analyzed through FTIR method (Fourier Transform Infrared). Emulsification activity over biosurfactant’s stability was analyzed from Box-Behnken model with software Minitab 17.0 among interactive factor of pH (2-12), temperature (40-121°C), dan salinity (2-10%). Also, Sand-Pack Column was been designed for simulation of the optimization result based on selected nitrogen source. The research indicated ammonium nitrate with concentration variety of 3,0 g/L (w/v) considered among selected nitrogen source, producing biosurfactant around 1558,33 ± 38,027 mg/L (p<0,05) with production rate of 17,80 mg/hr at 60 hours. In terms of CMC value, biosurfactant produced 946,43 mg/L with IFT minimum value as 26,20 dyne/cm. Based on functional groups which were been analyzed, the identification showed lipopeptide structure from biosurfactant. Stability of biosurfactant was well-preserved (Ei24 > 50%) at environment’s conditional range of 40-100 °C temperature, 2-10 pH dan 2-10% salinity. The simulation which also compared Additional Oil Recovery (%AOR) value between biosurfactant flooding and microbial flooding was successfully determined as 36,58% and 41,55%. Hence, consortium of bacteria which produced biosurfactant as stimulation’s result from ammonium nitrate, conducted any potential source for been implemented in MEOR technology.<p align="justify">