Enhancement of Biomass Production and Analysis of Protein Content in Chlorella vulgaris and Chlorella pyrenoidosa Cultivated in Bioslurry Medium Using Different Types of LEDs

• Main Author: Shafira (Nim : 11214023), Hanny
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/27710
• Institution: Institut Teknologi Bandung

Microalgae are photosynthetic organisms that have a potential to be developed as a promising alternative source of protein because of their high protein content and other valued nutritions that are beneficial for health. Along with their potential to produce high valued products, microalgae can also be used for the depollution of wastewater, called phycoremediation. Dairy manure wastewater that pass through anaerobic digestion process, or called bioslurry, is one of studied wastewater that can be used for microalgae growth medium since it is low-cost and provides inorganic nutrients for microalgae supplementation. In this study, Chlorella vulgaris and Chlorella pyrenoidosa are chosen microalgae species that have the ability in lowering excessive nutrients from the wastewater and simultaneously produce protein-rich biomass. Production of microalgae biomass can be enhanced by artificial lighting system using light-emitting diodes (LEDs) with specific wavelength range that captured by microalgae’s pigment for photosynthesis. The objectives of this study were to determine the best LED type for microalgae biomass production and analyse ortophosphate and ammonia reduction in bioslurry and protein content in both microalgae species. The experiment of this study was cultivation of C. pyrenoidosa and C. pyrenoidosa in bioslurry medium using red, blue, and white LED with intensity of 5000 lux. Method used in protein extraction is hot-TCA. This study shows that red LED gave best result (p<0,05) for biomass production in both species. Best biomass productivity cultivated in red LED was given by C. pyrenoidosa, which valued 0,114 ± 0,021 g L-1 day-1, while the productivity of C. pyrenoidosa was 0,098 ± 0,014 g L-1 day-1. Meanwhile, type of LEDs did not give results much differently in ortophosphate and ammonia reduction and protein content in microalgae. C. pyrenoidosa cultivated using three type of LEDs could lower 52-64% ortophosphate and 88-90% ammonia; while C. pyrenoidosa could lower 42-57% ortophosphate and 89-91% ammonia. Protein content in C. pyrenoidosa was ranged 8,3-8,5% (w/w), slightly higher (p<0,05) compared to protein content in C. pyrenoidosa (7,5-7,8% (w/w)).