COLONIZATION AND PERFORMANCE OF DIAZOTROPH ENDOPHYTIC BACTERIA ON PALM OIL (Elaeis guineensis Jacq L.) LEAVES
Excessive and continous use of inorganic fertilizer can lead to negative impact on the environtment qualities, therefore microbial biochemical approach which are more friendly need to be considered. The absorption of nitrogen elements has been widely known through the roots and then transported to l...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/27859 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Excessive and continous use of inorganic fertilizer can lead to negative impact on the environtment qualities, therefore microbial biochemical approach which are more friendly need to be considered. The absorption of nitrogen elements has been widely known through the roots and then transported to leaves before chlorophyll performing the works on the photosynthesis process. However, the discovery of many diazotroph endophytic bacteria in the leaves suggest that nitrogen is not only supplied through the soil but can be fixed by the diazotroph endophytic bacteria through the leaves. This study aims to evaluate the colonization and performance of the diazotroph endophytic bacteria on palm oil leaves. A total of 42 isolates of endophytic bacteria isolated by Mardiah (2014) were selected based on their ability to fix nitrogen qualitatively on nitrogen free medium and quantitatively based on Nessler reagent. One of the selected isolate with the highest nitrogen fixation capability is KSD2. The sequencing results showed that KSD2 isolate is closely related to Bacillus cereus. Colonization and performance evaluation of Bacillus cereus on palm oil seedlings were performed at 0, 2, and 4 weeks after inoculation. Treatments included Co (control/ untreated), Ta (inoculation via soil), and Da (inoculation via leaves) with 7 replications. Data were evaluated using SPSS program with Univariate Analysis of Variance (significance level> 95%). The colonization of Bacillus cereus were evaluated with PCR method approach using a specific primer (BCFomp1/ BCRomp1). PCR results showed that Bacillus cereus successfully colonized palm oil leaves as evidenced by the target sequences of 575 bp long. The result of nitrogen fixation showed that the inoculation through leaves was significantly higher 39.03% than control at week 4, while the soil inoculation method was not significantly different compared to control. Total chlorophyll has no statistically significant difference between both treatment compared with control. |
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